摘要
为改善克拉玛依油田的微生物强化原油开采,从克拉玛依油田废水中筛选出一株性能优良的生物表面活性剂生产菌株BC1。根据菌株BC1的理化指标和16S r DNA对其进行了属种信息鉴定,通过薄层层析(TLC)、傅里叶红外色谱(FT-IR)及高效液相色谱/质谱(HPLC-MS)对其产生物表面活性剂进行了分析,通过单因素摇瓶实验考察了发酵条件对菌株BC1发酵合成生物表面活性剂的影响,研究了生物表面活性剂的性能。研究结果表明,菌株BC1为铜绿假单胞菌属(Pseudomonas aeruginosa),其产生物表面活性剂主要成分为单鼠李糖脂Rh C10C10;在温度37℃、初始p H为8、初始葡萄糖浓度5 g/L的条件下持续发酵96 h后,用酸沉降法测得鼠李糖脂产量为0.788 g/L;菌株BC1产生物表面活性剂能将发酵液表面张力从72降至28.6 m N/m,对车用机油的乳化指数E24高达93%。人工油砂剥油实验中,发酵液、发酵离心上清液和鼠李糖脂粗品溶液对人工油砂的剥油率均达到74%以上。菌株P.aeruginosa BC1产生物表面活性剂在微生物强化采油等方面具有良好的应用潜能。
Strain BC1 with excellent performance of biosurfactant production was isolated from the wastewater of Karamay oilfield in order to improve the microbial enhanced oil recovery process. According to the physiological and biochemical indexes and 16S rDNA sequences analysis, the strain BC1 was classified. The biosurfactant produced by strain BC1 was identified by using thin-layer chromatography （TLC） , Fourier transform infrared spectrum （FT-IR） and high performance liquid chromatography-mass spectrometry （HPLC-MS） analysis. The effect of fermentation conditions on the biosurfactant produce of strain BCI was studied by single-factor shaking-flask culture experiment, and the performance of biosurfactant was researched. The results showed that strain BC1 belonged to Pseudomonas aeruginosa, and the key component of biosurfactant produced by strain BC1 was mono-rhamnolipid RhCt0C^o. The RLs yield was 0.788 g/L determined by acid precipitation method, under the condition of fermentation： 37℃ culture temperature, 8 initial pH value, 5 g/L glucose concentration, 96 h fermentation time. The biosurfactant production of strain BC1 could dramatically reduce the broth surface tension from 72 to 28.6 mN/m, and show high emulsification index against motor oil of 93%. In the oil stripping test, more than 74% of crude oil could be effectively stripped from the artificial oil sands by using the fermentation broth, fermentation supernatant and solution of crude rhamnolipid. The biosurfactant produced by strain P.aeruginosa BC1 had good application potential in microbial enhanced oil recovery and other aspects.
出处
《油田化学》
CAS
CSCD
北大核心
2016年第4期720-725,共6页
Oilfield Chemistry
基金
四川省科技创新苗子工程项目"利用生物燃料副产物甘油高密度发酵生产生物表面活性剂"(项目编号2015011)
西南石油大学科研启航计划项目(项目编号2014QHZ017)
四川省安全生产科技项目"氟化学品理化特性测试及氟化工工艺关键安全技术研究"(项目编号scaqjgjc_stp_20150018)
关键词
铜绿假单胞菌
鼠李糖脂
生物表面活性剂
表面活性
微生物强化采油
Pseudomonas aeruginosa
rhamnolipid
biosurfactant
surface activity
microbial enhanced oil recovery （ MEO R ）
