摘要
目的：初步研究不同生长环境和采收期对问荆药材中异槲皮苷的影响,并建立定性和定量的分析方法。方法：采用薄层色谱法,对问荆药材中异槲皮苷进行定性分析;采用HPLC法对问荆药材中异槲皮苷进行含量测定,Z O R B A X SB-C1 8（4.6 m m×2 5 0 m m,5μm）为色谱柱;流动相：乙腈-0.1%甲酸（1 5∶8 5）,流速为1.0 m L·m in-1;检测波长为354 nm;进样量为10μL。结果：采收期对问荆药材的薄层色谱和异槲皮苷含量均有较大的影响;薄层色谱斑点清晰,分离度良好;异槲皮苷在0.41~95.13μg·m L-1范围内与峰面积呈良好线性,r=0.9999,平均回收率为9 7.2%,R SD=1.3%。结论：每年6~8月是问荆药材的最佳采收期;所建立的方法简便,灵敏,重现性良好,可作为问荆药材的质量控制方法。
Objective： Study on different growth environment and harvest time on the quality of Equisetum arvens L . To establish methods for identification and determination of isoquercitrin in Equisetum arvens L. Methods： TLC was used to identify isoquercitrin in Equisetum arvens L, and the content was determine by HPLC, Agilent ZORBAX SB-C18（4. 6 mm × 250 mm,5 μm） column was used. Acetonitrile and 0. 1% Formic acid water-solution were used as mobile phase of liner elution, and the volume flow was at 1.0mL· min-1 , Uhraviolent determination wavelength was 354nm; and injection volume was 10μL Results： Different growth environment and harvest time influence obviously TLC and the content of isoquercitrin. TLC spots were clear, and sparated nicely. The linear range of isoquercitrin was 0. 41-95.13μg·mL-1. The average recovery in Equisetum arvens L was 97. 2%, RSD was 1.3%. Conclusion： June and August are the best harvest time. The method is believable for identitication and determination of isoquercitrin in Equisetum arvens L. with its simplicity, sensibility, repeatability.
出处
《中国药物评价》
2016年第6期485-488,共4页
Chinese Journal of Drug Evaluation
