摘要
【目的】研究不同林地土壤纤维素分解菌的数量及酶活性,为高效纤维素分解菌群的筛选提供参考。【方法】以缙云山的楠竹林、马尾松林和杉木林土壤为研究对象,测定其土壤养分含量、纤维素分解菌数量和纤维素酶活性,然后就三者之间的相关性进行分析。【结果】(1)杉木林地的土壤纤维素分解菌数量、相对酶活性、羧甲基纤维素酶活性(Cx)和滤纸酶活性(FPA)平均值均显著高于楠竹林地,分别是后者的9.70,1.24,2.80和2.81倍,且楠竹林地上述各指标均高于马尾松林地,分别是马尾松林地的1.36,1.15,1.03和1.07倍。(2)杉木林地和马尾松林地的Cx、FPA变化动态相似,Cx、FPA均在培养至5d时最高,之后略有下降;而楠竹林地的Cx、FPA则是在5d前平稳增长,之后迅速上升,培养至7d时最高。(3)相对酶活性、Cx、FPA两两呈极显著正相关,纤维素分解菌数量与相对酶活性、Cx、FPA呈极显著正相关,土壤纤维素分解菌数量、相对酶活性均与有机质、有效磷含量呈极显著正相关,Cx与有机质、全氮、全磷、有效磷呈显著正相关,FPA与有机质、全氮、全磷呈显著正相关,与有效磷呈显著负相关。【结论】杉木林地的纤维素分解菌活力较强,纤维素分解转化能力及碳循环速率高于楠竹林地和马尾松林地。
【Objective】This study investigated the number of cellulose degradation bacteria and enzyme activities in the different forest soils to provide reference for selecting efficient cellulolytic bacteria.【Method】Soil nutrition content,number of cellulose degradation bacteria,and celluase activities were determined in soils of bamboo,Masson pine and Chinese-fir forests in Jinyun Mountain and their correlations were analyzed.【Result】The average number of cellulose degradation bacteria,relative cellulose activities,and activities of CMCase and FPA in Chinese-fir forest were 9.70,1.24,2.80 and 2.81 times of those in bamboo forest.Those in bamboo forest were 1.36,1.15,1.03 and 1.07 times of those in Masson pine forest.The activities of CMCase and FPA in Chinese-fir forest were similar to those in bamboo.They all reached the maximum in the 5th day and then decreased slightly.The activities of CMCase and FPA in bamboo forest grew steadily within the first 5days and rapidly increased to the peaks at the 7th day.The relative activity,CMCase and FPA were significantly and positively correlated,while the number of cellulose degradation bacteria had significant and positive correlation with relative activity,CMCase and FPA.The number of cel-lulose degradation bacteria and the relative activity had significantly positive correlation with organic matter and available phosphorus.CMCase had significant and positive correlation with organic matter,total nitrogen,total phosphorus,and available phosphorus.FPA had significant and positive correlation with organic matter,total nitrogen and total phosphorus,while it had significant and negative correlation with available phosphorus.【Conclusion】Chinese-fir forest had higher celluase activities,cellulolytic conversion capacity and carbon cycle rate compared to bamboo forest and Masson pine forest.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2017年第1期83-89,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
重庆市应用开发计划项目(cstc2013yykfA80014)
关键词
纤维素分解菌
纤维素酶
林地
缙云山
cellulose degradation fungi
celluase activities
forest land
Jinyun Mountain
