摘要
试验旨在研究疆岳驴催乳素受体(prolactin receptor,PRLR)基因多态性及其与泌乳性状间的关系,探寻可用于疆岳驴选育的分子遗传标记。随机选取120头疆岳驴,对疆岳驴的泌乳性能指标进行测定,并运用PCR-SSCP方法对疆岳驴PRLR基因侧翼区进行多态性分析。结果显示,PRLR基因在疆岳驴群体中存在2种基因型:AA和AB,A为优势等位基因;测序结果显示,PRLR基因的碱基突变位置为g.29764584C>G,该突变为同义突变,没有导致编码的氨基酸发生改变;经关联分析,PRLR基因AB基因型个体平均日泌乳量显著高于AA基因型(P<0.05),AA基因型个体乳蛋白率显著高于AB基因型(P<0.05),在乳糖率上差异不显著(P>0.05)。疆岳驴PRLR基因突变与泌乳性状存在关联性,推测PRLR基因可以作为疆岳驴乳用型选育的分子遗传标记之一。
In this study,the single nucleotide polymorphism of prolactin receptor（PRLR）gene and its correlation with the lactation traits in Jiangyue donkeys was tested,and the molecular genetic marker of breeding Jiangyue donkeys was explored.120 individuals were analyzed association of PRLRgene polymorphism with lactation traits in Jiangyue donkeys.Genetic polymorphism of PRLRgene flanking region was detected by PCR-SSCP method.The results showed that there was 1mutation in PRLRgene and 2genotypes of AA and AB,and allele A was the dominant allele.The SNP g.29764584CG was identified of PRLRgene by sequencing,and it was a synonymous mutation,did not cause amino acid changes.Statistical results indicated that the SNP was significantly associated with average daily milk yield and protein percentage in Jiangyue donkey（P〈0.05）,there was no significant in the lactin percentage（P〉0.05）.The mutation might be as crucial DNA genetic marker for lactation performance selection in Jiangyue donkeys.
作者
毕兰舒
肖海霞
臧长江
何美升
托乎提.阿及德
帕热哈提江.吾甫尔
于茜
刘武军
BI Lan-shu XIAO Hai-xia ZANG Chang-jiang HE Mei-sheng TUOHUTI·Ajide PAREHATIJIANG·Wufuer YU Xi LIU Wu-jun(College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China Institute of Livestocks , Xinjiang Academy of Animal Science, Urumqi 830000, China)
出处
《中国畜牧兽医》
CAS
北大核心
2017年第1期180-185,共6页
China Animal Husbandry & Veterinary Medicine
基金
自治区高技术研究发展项目(201411106)
人社部留学人员科技活动资助启动类项目<驴泌乳相关功能基因表达调控机制研究>(新人社函〔2016〕438)
自治区科技兴新项目(2016023A06)
