摘要
以黄河三角洲滨海湿地广泛分布的甘蒙柽柳和中国柽柳为试材,用浓度为3.52%的地下天然盐水配制成含盐量分别为0.4%、0.8%、1.2%、1.6%、2.0%、2.4%溶液来模拟盐碱生境,经胁迫处理后,分别对不同盐胁迫下2种柽柳叶片的7个生理指标进行分析。结果表明:盐胁迫下2种柽柳叶片的含水量呈下降趋势,在土壤含盐量为1.6%时,甘蒙柽柳和中国柽柳叶片含水量与对照相比分别下降了8.61个百分点和20.07个百分点(P<0.01),此时叶表可见部分清晰盐结晶颗粒。盐胁迫下2种柽柳叶片内可溶性蛋白和可溶性糖含量均呈现出低-高-低的单峰曲线,并在含盐量为1.2%时分别达到最大值,经相关性分析,2种柽柳可溶性蛋白和可溶性糖均呈显著正相关。2种柽柳脯氨酸含量随着盐胁迫的增加呈现上升趋势,且与土壤含盐量呈显著正相关。2种柽柳的POD活性呈现先升高后下降的趋势,当含盐量为1.2%时,甘蒙柽柳和中国柽柳叶片POD活性到达最大值,分别为对照的3.5倍和3.6倍(P<0.01)。2种柽柳的SOD活性呈双峰曲线变化,甘蒙柽柳和中国柽柳叶片SOD活性在盐胁迫为0.8%时达到第一个峰值,此时活性分别为对照的2.7倍和3.4倍(P<0.01);在盐胁迫为1.6%时达到第二个峰值,活性分别为对照的2.9倍和5.1倍(P<0.01),其中中国柽柳的增幅均大于甘蒙柽柳。MDA含量随盐胁迫的增加先升高后降低,且盐胁迫后2种柽柳叶片中MDA含量均大于盐胁迫前。
Tamarix austromongolica and Tamarix chinensis were used as experimental materials, which widely distribute in the coastal wetlands of the Yellow River Delta. The saline - alkali habitat was simulated by irrigating different amounts of underground saline water with the concentration as 3.52 %. The contents of leaf water, soluble protein, soluble sugar, proline, malondialdehyde （MDA） and activities of peroxidase （POD） and superoxide dismutase （SOD） in Tamarix leaves were analyzed under different salt stress treatments. The results showed that the content of leaf water of Tamarix austromongolica and Tamarix chinensis both showed a decreasing trend under the salt stress. When the soil salinity reached to 1.6% , the content of leaf water of Tamarix austromongolica and Tamarix chinensis decreased by 8.61 and 20.07 percent point respectively com- pared with control （ P 〈 0.01 ） , and some clear salt crystalline particles appeared on the surface of leaves. The contents of soluble protein and soluble sugar of Tamarix austromongolica and Tamarix chinensis both changed as a single peak curve （ low - high - low） under the salt stress, and they reached the maximum when the sa- linity was 1.2%. For the two varieties of Tamarix, the content of soluble protein had a significant positive cor- relation with the content of soluble sugar by the correlation analysis. The content of proline of Tamarix aus- tromongolica and Tamarix chinensis showed a increasing trend with the increase of salinity, and had a signifi- cant positive correlation with the salinity. The activity of peroxidase （POD） of Tamarix austromongolica and Tamarix chinensis showed a trend of increasing firstly and then decreasing, and reached the maximum when the salinity was 1.2%, which were 3.5 times and 3.6 times of the CK respectively （P 〈 0.01 ）. The activity of superoxide dismutase （SOD） of Tamarix austromongolica and Tamarix chinensis changed as a bimodal curve, and reached the first maximum when the salinity was 0.8%, which were 2.7 times and 3.4 times of the CK respectively （P 〈0.01 ） ; and then reached the second maximum when the salinity was 1.6% , which were 2.9 times and 5.1 times of the CK respectively （ P 〈 0.01 ）. And the increase of Tamarix chinensis was greater than that of Tamarix austromongolica. The content of malondialdehyde （MDA） showed a trend of in- creasing firstly and then decreasing with the increase of salinity, and the content after the salt stress was higher than that before
出处
《山东农业科学》
2017年第1期53-58,共6页
Shandong Agricultural Sciences
基金
国家公益性行业(林业)科研专项(201404305)
山东省农业良种工程项目(2012LZ12-03)
山东省农业良种工程项目[鲁农良字(2016)6号]
关键词
盐碱生境
模拟
柽柳
盐胁迫
生理特性
Saline - alkali habitat
Simulation
Tamarix
Salt stress
Physiological characteristics
