摘要
对引进的‘Hort16A’猕猴桃进行组培再生体系建立,其中愈伤组织诱导、不定芽分化、不定芽增殖及生根培养分别采用L9(34)试验设计、单因素随机区组试验设计及L9(34)试验设计。结果表明:外植体在MS+0.05 mg/L NAA+0.3 mg/L ZT中培养20 d后诱导愈伤组织效果最好,诱导率达(97.78%±1.92%);继续培养14 d后,不定芽分化效果最佳,不定芽分化率达(84.44%±1.92%),不定芽芽高达(1.52±0.29)cm。猕猴桃不定芽在MS+1.5 mg/L ZT中培养30 d后,不定芽增殖旺盛效果最佳,增殖倍数达(3.67±0.33)倍,芽高达(2.93±0.12)cm。猕猴桃无菌苗在1/2 MS+0.7 mg/L IBA+0.1 g/L活性炭培养基中生根最佳,生根率、根长、根粗和平均每株生根数分别达(98.9%±1.9%)、(7.60±0.44)cm、(0.47±0.07)cm和(7.67±2.19)根。
The tissue culture regeneration system of kiwifruit ‘Hort16A’ （Actinidia chinensis ‘Hort16A’） introduced from abroad was established. The callus formation rate and adventitious bud differentiation rate were investigated using the L9（34） orthogonal experiment, proliferation of adventitious buds were further studied using single factor randomized block design of experiment, and the rooting of kiwifruit was researched using the L9（34） orthogonal experiment. The result indicated that the highest callus formation rate （97.78 ± 1.92%） was investigated from the explants in the MS medium contained 0.05 mg/L NAA and 0.3 mg/L ZT after culturing 20 d. That the differentiation rate and height of adventitious buds were 84.44 ± 1.92% and 1.52 ± 0.29 cm respectively were the best differentiation effect surveyed on the explants in this medium after another 14 d culturing. The biggest proliferation rate （3.67 ± 0.33） and height （2.93 ± 0.12 cm） of adventitious buds were surveyed on the buds in the MS medium contained 1.5 mg/L ZT after culturing 30 d. The best rooting that rooting ratio, root length, root width and number of roots generated per shoot were 98.9 ± 1.9%, 7.60 ± 0.44 cm, 0.47 ± 0.07 cm and 7.67 ± 2.19 respectively was investigated from the shoots in the 1/2 MS medium contained 0.7 mg/L IBA and 0.1 g/L activated carbon.
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2017年第1期54-60,共7页
Journal of Southwest Forestry University：Natural Sciences
基金
云南省林学一流学科建设经费(51600625)资助
国家林业局948项目(2012-4-62)资助
云南省高校林木遗传与繁育重点实验室研究生开放基金项目(YNGB201508)资助
关键词
猕猴桃
组织培养
愈伤组织
不定芽分化
生根培养
kiwifruit, tissue culture, callus, differentiation of adventitious buds, rooting culture
