摘要
以产zunyimycin A及BE-24566B类卤化天然化合物的Streptomyces sp.FJS31-2为研究对象,分别利用高分辨质谱(HRMS)和高效液相色谱(HPLC)为检测手段,对不同的碳源、氮源组合的培养基在不同的培养时间和萃取条件下获得的目标化合物进行定性和产量的分析。在基础培养基内添加10 g/L无水乙醇浸泡24 h后的腐殖酸,培养17 d,采取乙酸乙酯萃取可以使目标化合物产量提高。
Taking Streptomyces sp. FJS31-2 which produces zunyimycin A and BE-24566B halogenated natural compound as research object, the tar- get compound obtained from the fermentation medium with different carbon and nitrogen sources at different culture time and extraction conditions were qualitatively and quantitatively analyzed by high resolution mass spectrum （HRMS） and high performance liquid chromatography （HPLC）. Resuits showed when adding humic acid which soaked in the basic medium with 10 g/L absolute ethyl alcohol for 24 h, the target compound yield could be improved by ethyl acetate extraction at 17 d.
出处
《中国酿造》
CAS
北大核心
2017年第1期66-69,共4页
China Brewing
基金
国家自然科学基金(31160004
31460006)
贵州省科技学技术基金项目(黔科合J字[2010]2156
黔科合J字[2012]2348
黔科合SY字[2013]3013)
