摘要
目的：探讨长链非编码 RNA （lncRNA） H 19在肺癌患者血浆中的表达水平及其在肺癌诊断中的应用价值。方法收集2015年1月至2015年12月淮安市第一人民医院呼吸内科、放疗科收治的137例肺癌患者,90例肺良性病变,60例健康体检人群作为研究人群。通过荧光定量 PCR 检测 lncRNA H 19在上述人群血浆中的表达水平,统计分析肺癌血浆 lncRNA H 19表达水平与临床病理资料之间的关系,受试者特征（ROC）曲线和曲线下面积（AUC）评估血浆 lncRNA H19单独或与癌胚抗原（CEA）、鳞状细胞癌抗原（SCCA）和神经特异性烯醇化酶（NSE）联合诊断肺癌效能。结果肿瘤组患者血浆 lncRNA H19表达水平显著高于良性病变组（U =1525,P 〈0.001）及健康对照组（U=570,P〈0.001）,差异均有统计学意义,血浆lncRNA H19的表达水平与肿瘤大小（χ^2=6.177,P=0.017）、淋巴结转移（χ^2=5.579,P=0.024）密切相关。lncRNA H19、CEA、SCCA、NSE诊断肺癌的 AUC 分别为0.895、0.818、0.759、0.703。lncRNA H19与 CEA 联合,诊断肺癌AUC增加至0.949,敏感性为82.48%,特异性为94.67%,较CEA单独诊断有显著提高。结论肺癌患者血浆 lncRNA H 19水平显著高于肺良性病变患者及健康对照人群,与临床病理学特征相关,在肺癌诊断中具有一定价值,可辅助传统肿瘤标志物用于肺癌诊断及病情评估。
Objective The study was performed to evaluate the clinical values of plasma long non-coding RNA （lncRNA） H19 as a diagnostic biomarker for patients with lung cancer. Methods Quantitative real-time polymerase chain reaction （qRT-PCR） was used to measure the expression of lncRNA H19 in plasma from 137 patients with lung cancer,90 patients with benign lung diseases,and 60 healthy controls.The area under the receiver operating characteristic（AUC）was used to evaluate the diagnostic performance of lncRNA H19 and compared it with common tumor markers,such as carcinoembryonic antigen （CEA）,squamous cell carcinoma antigen （SCCA）,and neuron-specific enolase（NSE）.The association between plasma H19 expression and clinicopathological characteristics were also analyzed.Results Plasma levels of lncRNA H19 were significantly up-regulated in lung cancer patients compared with benign lung diseases group（U=1 525,P〈0.001）,and normal controls group（ U=570,P〈0.001）.Overexpression of lncRNA H19 was closely associated with larger tumor size（χ^2=6.177,P=0.017）,and more lymph node metastasis（χ^2=5.579,P =0.024）.The AUC of lncRNA H19,CEA,SCCA,NSE were 0.895,0.818,0.759,and 0.703,respectively.Moreover,use of lncRNA H19 and CEA in combination could provide a more effective diagnosis performance（AUC,0.949,sensitivity, 82.48%,specificity,94.67%,P〈0.001）.Conclusions Plasma lncRNA H19 may be a useful biomarker＆amp;nbsp;for detection of lung cancer.The combination of lncRNA H19 with CEA was more effective for lung cancer diagnosis.
出处
《国际呼吸杂志》
2016年第23期1809-1813,共5页
International Journal of Respiration
关键词
肺肿瘤
长链非编码RNA
肿瘤标志
诊断
Lung neoplasms
Long non-coding RNA
Tumor biomarker
Diagnosis
