摘要
为明确取食不同寄主植物的西花蓟马和花蓟马对CO_2浓度升高的响应机制,分别选取四季豆、茼蒿和辣椒寄主,分析不同CO_2浓度对2种蓟马成虫体内解毒酶和保护酶活性的影响。结果表明,2种蓟马的羧酸酯酶(Car E)、乙酰胆碱酯酶(Ach E)、微粒体多功能氧化酶(MFO)、过氧化氢酶(CAT)和过氧化物酶(POD)活性均随着CO_2浓度的升高而增强。与对照相比,高CO_2浓度下西花蓟马取食四季豆时Car E和POD活性分别升高了21.70%和40.16%,取食茼蒿时Car E、MFO和POD活性分别升高了40.11%、6.73%和43.85%,而以辣椒为寄主时超氧化物歧化酶(SOD)活性降低了57.56%;在800μL/L高CO_2浓度下,花蓟马的Car E、Ach E和MFO活性均略有升高;四季豆、茼蒿和辣椒饲养的花蓟马SOD活性分别下降了26.84%、52.16%和53.71%。研究表明,2种蓟马均能通过调节体内的生理酶活性来适应环境的变化,且西花蓟马的适应能力强于花蓟马。
To clarify the response mechanisms of Frankliniella occidentalis and F. intonsa to elevated carbon dioxide（ CO_2）,the activities of detoxifying enzymes and protective enzymes of thrips feeding on kidney bean, crown daisy and pepper were studied. The results showed that the activities of caboxylesterase（ Car E）,acetylcholinesterase（ Ach E）,microsomal mixed-function oxidases（ MFO）,catalase（ CAT） and peroxidase（ POD） of two thrips were enhanced with increasing CO_2 concentration.Compared with the control,the activities of Car E and POD of F. occidentalis fed on kidney bean foliages under elevated CO_2（ 800 μL/L） conditions increased by 21. 70% and 40. 16%,respectively. The activities of Car E,MFO and POD of the populations fed on crown daisy foliages increased by 40. 11%,6. 73% and 43. 85%,respectively. However,the activities of superoxide dismutase（ SOD） in F.occidentalis fed on pepper leaves decreased by 57. 56%. When F. intonsa grew under CO_2 of 800 μL / L,the activities of detoxifying enzymes increased slightly,and the activities of SOD of F. intonsa fed on the three host plants decreased by 26. 84%,52. 16% and 53. 71%,respectively. The results indicated that both of F. occidentalis and F. intonsa could adjust the activities of physiological enzymes to environmental alteration,and the adaptability of F. occidentalis was higher than that of F. intonsa.
出处
《植物保护学报》
CAS
CSCD
北大核心
2017年第1期45-53,共9页
Journal of Plant Protection
基金
国家自然科学基金(31260450,30860069)
关键词
CO_2浓度
西花蓟马
花蓟马
生理酶
elevated CO_2
Frankliniella occidentalis
Frankliniella intonsa
physiological enzyme
