摘要
通过建立Ⅱ型糖尿病小鼠模型,以血糖值的变化确定南瓜多糖、葛根黄酮、普洱茶褐素提取物降血糖的最佳剂量。在该基础上,通过对其空腹血糖值(FBG)、胰岛素(INS)、甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)的测定,表明3种提取物单一组分及两两联合对Ⅱ型糖尿病小鼠糖脂代谢紊乱均有明显的改善作用,在FBG、INS、IR、HDL、LDL指标上联合组显著优于单一组分(P<0.05)。析因分析结果表明,当3种物质两两联合时具有交互作用;协同试验发现,南瓜多糖与葛根黄酮联合或与茶褐素联合在降低TG含量上均与多糖组差异显著(P<0.05);茶褐素与葛根黄酮联合在降低FBG、TC、LDL含量上效果显著优于黄酮组,在TG、INS方面效果显著优于茶褐素组和黄酮组(P<0.05)。分子机制试验表明,3种物质单一组分和两两联合在PI3K/Akt蛋白通路表达方面均有增强作用。
In this paper,the type II diabetes model was established and the optimal dose of the pumpkin polysaccharide,pueraria flavonoids and pu-erh theabrowns were determined by measured blood glucose.On the basis,the fasting blood glucose（FBG）,insulin（INS）,insulin resistance（IR）,high density lipoprotein（HDL）and low density lipoprotein（LDL）were also investigated.The FBG,INS,IR,HDL,LDL combined group was significantly better than the single component（P0.05）.The results revealed that the combinations of pumpkin polysaccharide and pu-erh theabrowns,pueraria flavonoids and pu-erh theabrowns were effective.However,the pumpkin polysaccharide factorial analyses showed that the interactions of the three substances were interactive.The synergistic experiment showed that pumpkin polysaccharide combined with Pueraria lobata flavonoids or combination with tea-flavonoid significantly reduced TG content（P0.05）.The combination of theaflavin and pueraria flavonoids in reducing the content of FBG,TC,LDL was significantly better than flavonoids,and the effect of TG and INS was better than that of thepueraria flavonoids and pu-erh theabrowns group（P0.05）.The molecular mechanism experiments showed that a single one and two of the three components played an important role in the expression of PI3K/Akt protein pathway.
出处
《食品与机械》
CSCD
北大核心
2016年第12期142-147,共6页
Food and Machinery
基金
天津市科技支撑计划项目(编号:13ZCZDNC01800)
天津市农业科技成果转化项目(编号:14ZXNZNC00036)
