摘要
采用酶解结合物理法对雨生红球藻的破壁工艺进行了研究,以破壁孢子率为评价指标,通过正交试验筛选酶解条件,对比试验进行验证。试验结果表明,雨生红球藻的最佳破壁工艺为:选用复合纤维素酶对雨生红球藻进行酶解处理,酶添加量0.5%,酶解温度45℃,酶解时间5 h,酶解后结合高压均质法进行破壁,均质压力40 MPa,均质2次。在此工艺条件下,雨生红球藻的破壁孢子率较原工艺提高18.07%。酶解结合高压均质法用于雨生红球藻粉破壁具有效率高、成本低的优点,适用于工业化生产。
Tostudythe cell wall-breakingprocess byenzyme combined with physical method for Haematococcus pluvialis. Methods：Cell wall-breaking rate was as the evaluation index.Orthogonal test method was used to concern enzymolysis conditions, and verified by contrast experiment. Results： The enzymolysis conditions identified as： composite cellulose enzyme, adding amount of 0.5%,enzyme solution temperature 45 ℃, enzymolysis time 5 h,homogeneous pressure 40 Mpa, homogeneous 2 times. Compared with the original method, cell wall-breaking rate of new method increased 18.07%.Conclusion： Enzyme combined with physical method is high efficiency, low cost for breaking Haematococcus pluvialiscell wall, and is suitable for industrialized production.
出处
《食品工程》
2016年第4期38-40,共3页
Food Engineering
关键词
雨生红球藻
虾青素
酶解
高压均质
破壁
haematococcus pluvialis
astaxanthin
enzymolysis
high pressure homogeneous
cell disruption
