摘要
本研究以内蒙古达茂旗牧区牛乳中分离的16株乳酸菌为研究对象,采用纸片扩散法对氧氟沙星、环丙沙星、诺氟沙星和链霉素四种抗菌药进行耐药性测定。实验结果表明:93.75%的菌株对氧氟沙星和链霉素表现为耐药,100%的菌株对环丙沙星和诺氟沙星表现为耐药;耐药菌株经PCR扩增、gyr A基因编码的氨基酸序列分析,发现有1株乳酸菌的耐药性与gyr A基因突变有关。
The research subject of this study was sixteen strains of lactobacillus which separated from milk of Damao pastoral areas of Inner Mongolia, and paper diffusion method was used to test the drug resistance of four kinds of antibacterial drugs of ofloxacin, ciprofloxacin, norfloxacin, and streptomycin. The results showed that 93.75 % of sixteen strains of lactobacillus were resistant to ofioxacin and streptomycin, and 100% of sixteen strains of lactobacillus were resistant to ciprofloxacin and norfloxacin.The results of PCR amplification and amino acid sequence analysis for all drug resistance lactobacillus showed that the resistance of one strains of lactobacillus was related to mutation of gyrA gene.
作者
吕耀龙
李少英
赵春杰
包丹丹
LV Yao-long LI Shao-ying ZHAO Chun-jie BAO Dan-dan(Vocational and Technical College of Inner Mongolia Agricultural University, Baotou 014109, China Food Science and Engineering College of Inner Mongolia Agricultural University, Hohhot 010018, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第3期166-169,185,共5页
Science and Technology of Food Industry
基金
内蒙古高等学校科学研究项目(NJ09059)
国家自然科学基金项目(31060014)
关键词
乳酸菌
耐药性检测
gyrA基因分析
Lactobacillus
drug resistance testing
gene analysis of gyrA
