摘要
目的建立一测多评法测定祛白合剂中丹参多种指标成分的含量。方法以阿魏酸为内参物,确定丹参素钠、原儿茶酸、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸B相对于阿魏酸的相对校正因子,比较一测多评法与外标法含量测定结果的差异,评价一测多评法的可行性和准确性。结果祛白合剂中丹参各指标成分的相对校正因子重现性良好,一测多评法和外标法测定结果差异无统计学意义。结论本实验所建立的一测多评法简单方便,可用于祛白合剂的质量评价。
Objective To quantitatively analyze the multi-components by single marker （QAMS） determination method in Qubai mixture with high pressure liquid chromatography. Methods With ferulic acid as the internal standard, we assessed the relative correcting factors of sodium danshensu, protocatechuic acid, protocatechuic aldehyde, caffeic acid, rosemary acid and salvianolic acid B. We compared the content difference determined by QAMS and external standard methods, evaluated the feasibility and the accuracy of QAMS method. Results There was no significant difference between QAMS and external standard method, and the relative correcting factors of each index component exhibited good reproducibility. Conclusion The QAMS by RP-HPLC in our study allows for a simple, useful and reliable method for the quality control of Qubai mixture.
作者
熊鑫
饶俊珍
王富乾
程璐
XIONG Xin RAO Jun-zhen WANG Fu-qian CHENG Lu(Department of Pharmacy, Wuhan First Hospital, Wuhan 43002)
出处
《中南药学》
CAS
2017年第1期102-106,共5页
Central South Pharmacy
关键词
祛白合剂
一测多评法
相对校正因子
阿魏酸
丹参
Qubai mixture
quantitative analysis of multi-components by single marker
relative correction fac- tor
ferulic acid
Salvia miItiorrhiza
