摘要
目的探讨不同强度运动处方对老年肥胖大鼠内脏脂肪组织特异性丝氨酸蛋白酶抑制剂(Vaspin)mRNA、蛋白表达和血Vaspin浓度的影响。方法选取雄性清洁级SD幼鼠,按照生长发育期、壮年期和老年期三个阶段增肥,建立老年肥胖大鼠模型,选取6只自然生长老年大鼠为空白对照组,选取18只肥胖老年大鼠为实验组,随机分为3组,每组6只,分别为肥胖对照组、低强度运动组、中强度运动组。运动处方分别为12 m/min×15 min、4组/次、组间休息5 min、60 min/次;15 m/min×15 min、4组/次、组间休息5 min、60 min/次。跑台坡度均为0°,5次/周,连续8周。运动干预后测内脏脂肪组织Vaspin mRNA表达采用qRT-PCR、测蛋白表达采用WB、测血Vaspin浓度采用ELISA。结果运动干预后,(1)Vaspin mRNA表达(×10~3):肥胖对照组(25.761±11.581)高于空白对照组(12.161±5.648)(P<0.01),低强度运动组(10.482±2.668)、中强度运动组(13.478±5.115)低于肥胖对照组(P<0.01),中强度运动组高于低强度运动组,但差异无统计学意义(P>0.05)。(2)Vaspin蛋白表达:肥胖对照组高于空白对照组(P<0.01),低强度运动组低于肥胖对照组(P<0.05),但中强度运动组高于肥胖对照组(P<0.01)。(3)血Vaspin浓度:肥胖对照组[(25.000±3.767)ng/m L]高于空白对照组[(23.765±3.045)ng/m L],低强度运动组[(25.637±5.240)ng/mL]和中强度运动组[(26.422±3.4175)ng/mL]高于肥胖对照组,但差异均无统计学意义(P>0.05)。(4)血糖、胰岛素敏感性指数:肥胖对照组的血糖[(17.930±6.094)mg/dL]高于空白对照组[(11.636±3.568)mg/dL](P<0.01),胰岛素敏感性指数肥胖对照组(0.369±0.017)低于空白对照组(0.394±0.019),但差异无统计学意义(P>0.05);低[(13.362±1.819)mg/dL]、中强度运动组[(12.842±1.905)mg/dL]血糖低于肥胖对照组(P<0.05),而胰岛素敏感性指数[低(0.385±0.018),中(0.396±0.015)]高于肥胖对照组(P<0.01)。结论老年肥胖大鼠内脏脂肪的Vaspin mRNA表达、蛋白表达和血浆含量偏高。低强度运动可降低Vaspin mRNA基因表达量和蛋白表达,运动强度增加又提高Vaspin mRNA表达和蛋白表达以及血浆Vaspin水平。
Objective To investigate effects of different intensity exercise prescription on visceral adipose tissue-derived serine protease inhibitor（ Vaspin） mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats. Methods SD rats as three stage of fattening divided the growth period,middle-aged and elderly,to establish rats model of elderly obesity,6 natural growing aged rats were selected as blank control group,18 elderly obese rats were randomly divided into 3 groups,6 rats in each group： obese control group,low intensity exercise group,moderate intensity exercise group. Exercise prescription were 12 m /min × 15 min for each group,4groups / times,rest between groups 5min,60 min/time. 15 m/min×15 min for each group,4 groups / times, rest between groups 5min, 60 min / time. Vaspin mRNA expression was measured by qRT-PCR and Vaspin protein expression was measured by WB in visceral adipose tissue and plasma Vaspin concentration was measured by ELISA after exercise. Results After exercise intervention,（1） Vaspin mRNA（×103） expression：Obese control group（25.761±11.581） was higher than the control group（12.161±5.648）.（P0.01）,low intensity exercise group and moderate intensity exercise group（（10.482±2.668）,（13.478±5.115）） were lower than obese control group （P0.01）,moderate intensity exercise group was higher than low intensity exercise group,but the difference was not statistically significant （P0.05）.（2） Vaspin protein expression： Obese control group was higher than the control group （P0.01）,low intensity exercise group was lower than obese control group （P0.05）,but moderate intensity exercise group was higher than obese control group （P0.01）.（3） Plasma Vaspin concentration： Obese control group（（25.000±3.767） ng/mL） was higher than the control group（（23.765±3.045） ng/m L）,low intensity exercise group and moderate intensity exercise group（（25.637±5.240） ng/mL and （26.422±3.4175） ng/mL） was higher than obese control group,but the difference were not statistically significant（ P 0. 05）.（ 4） Blood glucose and insulin sensitivity index： Blood glucose of obese control group was higher than the control group（ P 0. 01）. Insulin sensitivity index of obese control group was lower than the control group,but the difference was not statistically significant（ P〈0. 05）. Blood glucose of low and moderate intensity exercise groups were lower than obese control group（ P〈0. 05）,but insulin sensitivity index of low and moderate intensity exercise groups were higher than obese control group（ P〈0. 01）.Conclusion Vaspin mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats are higher. Low intensity exercise can reduce Vaspin mRNA and protein expression,but increase exercise intensity may improve Vaspin mRNA and protein expression in visceral adipose tissue and plasma Vaspin concentration of elderly obese rats.
作者
林潺
柏友萍
孙明明
张皓月
苏敏
崔嘉琴
宋威巍
吴琳
Lin Chan Bai Youping Sun Mingming Zhang Haoyue Su Min Cui Jiaqin Song Weiwei Wu Lin(College of Physical Education,Anhui Normal Universit)
出处
《卫生研究》
CAS
CSCD
北大核心
2017年第1期70-77,共8页
Journal of Hygiene Research
基金
安徽师范大学研究生科研创新与实践重点项目(No.2015cxsj011zd)
