摘要
目的:通过研究蝉蜕不同提取部位对小鼠、豚鼠的止咳祛痰平喘作用,对蝉蜕有效部位进行筛选。方法:蝉蜕使用70%乙醇回流提取,所得浸膏依次用石油醚、乙酸乙酯、正丁醇萃取得各部分浸膏,受试动物随机分为对照组、阳性药组及不同提取部位组,采用小鼠氨水引咳法筛选止咳有效部位,小鼠酚红气管段法筛选祛痰有效部位,豚鼠整体药物引喘法筛选出平喘有效部位。结果:与对照组比较,蝉蜕70%乙醇提取物水部位有明显止咳作用(P<0.05),正丁醇部位有祛痰作用(P<0.01),乙酸乙酯、水部位具有明显平喘作用(P<0.01)。结论:蝉蜕的止咳有效部位是水部位,祛痰部位为正丁醇部位,平喘部位是乙酸乙酯及水部位。
Objective： To compare the cough relieving, sputum reducingand asthma relieving actionsof different of Cicadae P ts. Methods： The antitussive, expectorant and anti-asthmatic effects were observed by using cough mice caused by ammonia, tracheal phenol red test and asthma guinea-pigs caused by acetylcholine chloride and histamine phosphate. Results： Water decoction and water fraction showed acough-relieving action, n-butanol fraction exhibited an expectorant effect, and water decoction, ethyl acetate extract and water fraction showed ananti-asthma action. Conclusion： The water fraction is the active part on cough relieving, n-butanol part is the effective part on reducing sputum, and the water fraction and ethyl acetate extractare active parts for relieving asthma.
作者
江泓
杨炀
陈胡兰
傅超美
邓赟
张梅
JIANG Hong YANG Yang CHEN Hulan FU Chaomei DENG Yun ZHANG Mei(School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China)
出处
《中国现代中药》
CAS
2017年第1期56-59,共4页
Modern Chinese Medicine
基金
四川省科技支撑计划项目(2013SZZ016)
四川省科技厅"中药药效物质基础研究与创新中药发现四川省青年科技创新研究团队"项目(2016TD0006)
关键词
蝉蜕
止咳
祛痰
平喘
有效部位
Cicadae Periostracum
antitussive effect
expectorant effect
anti-asthmatic effect
effective part
