摘要
为了研究腺苷酸激酶4(AK4)在大鼠胚胎内胚层前期细胞(XEN-P)分化过程中所起的作用,试验采用蛋白免疫印迹和RNA干扰的方法,检测了AK4在XEN-P细胞中的表达。结果表明:AK4的表达随着XEN-P细胞的分化呈逐渐上升的趋势;干扰AK4的表达后对XEN-P细胞的形态和细胞周期均无影响。说明AK4的表达与XEN-P细胞的分化程度呈正相关。
To study the role of adenylate Kinase 4 （AK4） in the differentiation process of rat extraembryonic endoderm precursor （XEN - P） cells, the methods of Western- blot and RNA interference （RNAi） were applied to detect AK4 expression in the XEN -P cells. The results showed that AK4 expression exhibited a gradual upward trend with the differentiation of XEN - P ceils, and the AK4 expression had no effect on the morphology and cell cycle of XEN - P cells after using RNAi. The results indicate that AK4 expression is positively correlated with differen- tiation grade of XEN - P cells.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2017年第2期31-33,289,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
黑龙江省教育厅海外学人科研资助项目(1253HQ013)
