摘要
昆虫小分子热激蛋白(smHsp)是分子质量在12~43 k Da、具有分子伴侣活性的应激蛋白,可以在环境胁迫下帮助蛋白质折叠和转运。本文为明确舞毒蛾的smHsp基因特性及对杀虫剂甲萘威胁迫的响应,从舞毒蛾无参照全转录本文库中鉴定获得6个小分子热激蛋白家族基因,全长基因开放阅读框为444~567 bp,编码147~188个氨基酸,理论等电点为5.58~6.17。根据分子质量大小分别命名为Ld Hsp17.0、Ld Hsp18.7、Ld Hsp19.1、Ld Hsp20.3、Ld Hsp21.3和Ld Hsp21.4。亚致死剂量(LC_5、LC_(10)和LC_(30))的甲萘威处理可诱导舞毒蛾smHsp家族中Ld Hsp20.3、Ld Hsp19.1和Ld Hsp17.0上调表达,但抑制了Ld Hsp21.4、Ld Hsp21.3和Ld Hsp18.7的表达,且甲萘威处理对smHsp基因表达量的影响存在质量浓度和剂量效应。以上结果为进一步研究舞毒蛾小分子热激蛋白功能及其参与对杀虫剂抗性的机制提供了基础。
Small heat shock proteins of insect with molecular weight from 12 to 43 k Da are stress proteins with molecular chaperone activity and can assist protein folding and transporting under environmental stress. In present study,the full length of six smH sp c DNA was identified from Lymantria dispar transcriptome. The open reading frames（ ORF） of smH sps were from 444 to 567 bp encoding proteins of 147-188 amino acid residues with the theoretical p I of 5. 58- 6. 17. These smH sps were named as Ld Hsp17. 0,Ld Hsp18. 7,Ld Hsp19. 1,Ld Hsp20. 3,Ld Hsp21. 3 and Ld Hsp21. 4 according to their molecular mass. The effects of sublethal concentrations（ LC_5,LC_（10） and LC_（30）） of carbaryl on smH sp gene expression in L. dispar larvae were analyzed using real-time RT-PCR technology. The results showed that the expressions of Ld Hsp20. 3,Ld Hsp19. 1 and Ld Hsp17. 0 were induced while those of Ld Hsp21. 4, Ld Hsp21. 3 and Ld Hsp18. 7 were inhibited under three sublethal concentrations of carbaryl. The smH sp gene expressions were in concentration- and time-dependent way. Our study provides the basis for further studying the functions of smH sp and their roles in insecticide resistance in L. dispar.
作者
刘鹏
孙丽丽
张琪慧
曹传旺
LIU Peng SUN Li-li ZHANG Qi-hui CAO Chuan-wang.(School of Forestry, Northeast Forestry University, Harbin, Heilongjiang, 150040, P. R. China.)
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2017年第1期78-84,共7页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(31570642
31101676)
黑龙江省自然科学基金面上项目(C201409)
