摘要
为探究奶牛静脉血及脐静脉血中脂联素(adiponectin)、瘦素(leptin)、胰岛素样生长因子1(IGF-1)与犊牛初生体质量间的相关性。选取规模化养殖场54头正常分娩奶牛,按犊牛初生体质量划分为A(≤40kg),B(40~45 kg),C(≥45 kg)3组,采集分娩奶牛静脉血及脐静脉血,用ELISA方法检测静脉血中脂联素、瘦素和IGF-1的表达水平,分析其间的相关性及与犊牛初生体质量的相关性。结果表明,奶牛静脉血脂联素、瘦素和IGF-1表达水平两两之间均呈极显著正相关(P<0.01),但奶牛静脉血脂联素、瘦素和IGF-1表达水平与犊牛初生体质量相关性均不显著(P>0.05);脐静脉血脂联素、瘦素和IGF-1表达水平两两之间均呈极显著正相关(P<0.01),脐静脉血脂联素、瘦素和IGF-1表达水平均随犊牛初生体质量增加而逐渐升高,且分别与犊牛初生体质量呈极显著正相关(P<0.01);奶牛静脉血脂联素、瘦素和IGF-1表达水平均极显著高于脐静脉血(P<0.01),而奶牛静脉血脂联素、瘦素和IGF-1的表达水平与脐静脉血中表达水平相关性均不显著(P>0.05)。据此得出,奶牛静脉血和脐静脉血中均有脂联素、瘦素和IGF-1表达,奶牛静脉血中及脐静脉血中脂联素、瘦素和IGF-1均各自相互协调发挥作用;奶牛静脉血脂联素、瘦素和IGF-1均对犊牛初生体质量影响不显著,但脐静脉血脂联素、瘦素和IGF-1均对犊牛初生体质量影响极显著,该结果可为进一步研究脂联素、瘦素和IGF-1对犊牛初生体质量的影响机制奠定基础。
In order to explore the correlation between calf birth weights and expression level of adiponectin,leptin,IGF-1 in cow＇s venous and cord venous blood. Fifty-four healthy Chinese Holstein cows that experienced normal natural birth were used,and were divided into 3 groups based on calf birth weights（A,≤40 kg; B,40 - 45 kg; C,≥45 kg）. The cow＇s venous blood and cord venous blood was collected. ELISA was used to evaluate the expressionlevels of adiponectin,leptin and IGF-1 to analyze the correlation among them as well as correlation with calf birth weights. It was shown that there were significant（P〈0. 01） correlations among adiponectin,leptin and IGF-1 in cow＇s venous blood,while these indexes showed no significant（P〉0. 05） correlations with calf birth weights. In cow＇s cord venous blood,adiponectin,leptin,and IGF-1 were significantly（P〈0. 01） correlated,and adiponectin,leptin,and IGF-1 in cord venous blood as whole were positively correlated with calf birth weights,and was significantly（P〈0. 01） correlated with calf birth weights individually. Adiponectin,leptin,and IGF-1 expression levels in venous blood were significantly（P〈0. 01） higher than those in cord venous blood,and were not significantly（P〉0. 05） correlated with those in cord venous blood. The results showed that there were adiponectin,leptin,and IGF-1 in venous blood and cord venous blood. Adiponectin,leptin,and IGF-1 in venous and cord venous blood were potentially inter-regulated with each other. Adiponectin,leptin,and IGF-1 expression levels in venous blood were not significantly（P〈0. 05） correlated with calf birth weights,while adiponectin,leptin,and IGF-1 expression levels in cord venous blood were significantly（P〈0. 01） correlated with calf birth weights,respectively.
出处
《浙江农业学报》
CSCD
北大核心
2017年第1期37-43,共7页
Acta Agriculturae Zhejiangensis
基金
四川省教育厅重点项目(15ZA0024)
四川省科技厅科技支撑项目(2011NZ0060
2013NZ0032)
四川农业大学双支计划
