摘要
目的探讨抗核抗体(ANA)及抗着丝点抗体(ACA)检测对小细胞肺癌(SCLC)患者的诊断意义。方法对江西省南昌市新建区中医院和南昌大学第二附属医院2015年1月-2016年5月间符合纳入标准的66例SCLC患者、116例其他肿瘤患者及40例健康体检者血清作为研究对象,并分别以人喉癌上皮细胞-20-10(HEp-20-10)和人喉癌上皮细胞-2(HEp-2)作基质采用间接免疫荧光法(IIF)检测ANA、ACA,并对结果进行回顾性分析。结果与非小细胞肺癌(NSCLC)相比,SCLC血清中ANA阳性率显著增加(P<0.01);SCLC患者ANA检出率与肝癌患者相比,差异无统计学意义(P>0.01);SCLC与各病例对照组相比,ACA检出率明显增多,差异均具有统计学意义(P<0.01);各肺癌组血清中ANA和ACA的检出率与健康对照组相比均显著性升高(P<0.01);分别采用HEp-20-10和HEp-2作为基质的IIF法对血清ACA进行检测,SCLC患者差异无统计学意义(P=0.824),Kappa一致性分析,P<0.01,有显著性差异,但Kappa=0.333,一致性较差;联合2种不同基质检测SCLC患者结果显示其阳性率显著提高(P<0.05),但从临床评价指标可见:基质为HEp-20-10的IIF检测结果的临床意义更显著。结论 ACA的检测对SCLC患者的临床诊断及鉴别诊断具有重要的临床意义,采用HEp-20-10细胞株检测ACA抗体更有助于SCLC患者检出率。
Objective To explore diagnostic significance and clinical evaluation of the antinuclear antibody（ANA） and anticentromere antibody（ACA） detection in patients with small cell lung cancer（SCLC）. Methods 66 cases of SCLC patients,116 cases of patients with other tumors and 40 cases of healthy people of serum as the research objects who come from Xin Jianxian Traditional Chinese Medicine Hospital and the Second Affiliated Hospital of Nanchang University from January 2015 to May 2016.The result of ANA and ACA was determined using human laryngeal epithelial cells-20-10（HEp-20-10） and human laryngeal epithelial cells-2（HEp-2） as the matrix by indirect immunofluorescence（IIF）,and the results were retrospectively analyzed. Results Compared with non-small cell lung cancer（NSCLC）,ANA in serum positive rate of SCLC significantly increased（P0.01）; ANA of SCLC patients was compared with liver cancer patients,there were no statistically significant difference（P0.01）;SCLC compared with the cases in the control group,the ACA detection rate were significantly increased,the differences were statistically significant（P0.01）;Each group of ANA in serum and lung cancer detection rate of ACA in comparison with healthy controls were significantly higher（P0.01）;The HEp-10-20 and HEp-2 as the matrix of the IIF method to test the serum ACA respectively,there were no statistically significant differences（P=0.824） to SCLC patients,Kappa consistency analysis,P0.01,there is a significant difference,but the Kappa=0.333,poor consistency;Combined two different substrate detection in patients with SCLC results showed that the positive rate significantly increased（P0.05）,but from the clinical evaluation indexes： matrix for HEp-20-10 IIF the clinical significance of the detection results are more significant. Conclusion The diagnosis and differential diagnosis of ACA detection in patients with SCLC has important clinical significance,using HEp-20-10 cell lines antibody detection of ACA will be having a better deterministic rate in patients with SCLC.
出处
《江西医药》
CAS
2016年第12期1326-1329,共4页
Jiangxi Medical Journal
基金
江西省科技厅社发项目(编号:2015BBG70154)
江西省科技厅重点科技成果转移转化计划(编号:20151BBI90047)
江西省卫生厅科研计划支持项目(编号:20133075
20151072)
关键词
小细胞肺癌
抗着丝点抗体
抗核抗体
人喉癌上皮细胞-20-10
人喉癌上皮细胞-2
Small Cell Lung Cancer
Anti-centromere Antibody
Antinuclear Antibody
Human laryngeal epithelial cells-2010
Human laryngeal epithelial cells-2
