摘要
目的:探讨长链非编码RNA SPRY4-IT1(Lnc RNA)对髓母细胞瘤增殖及侵袭转移的影响。方法:髓母细胞瘤Daoy细胞分为对照组和si-SPRY4-IT1组,分别利用脂质体Lipofectamine 2000将阴性对照荧光序列和SPRY4-IT1-si RNA转入细胞中,采用Real-time PCR检测转染后各组细胞中SPRY4-IT1的表达情况,CCK-8实验及平板克隆形成实验检测细胞增殖能力的变化,以细胞体外侵袭、迁移实验分别检测细胞侵袭、迁移能力的变化,Western blot检测SPRY4-IT1对基质金属蛋白酶(MMP)-2和MMP-9蛋白的影响。结果:si-SPRY4-IT1组SPRY4-IT1 m RNA表达水平、细胞体外增殖能力、细胞侵袭、迁移能力、细胞MMP-2蛋白表达均较对照组明显降低(P<0.05),而MMP-9蛋白表达未见明显变化。结论:干扰长链非编码RNA SPRY4-IT1在髓母细胞瘤Daoy细胞中的表达能显著抑制细胞的增殖、侵袭和迁移能力。
Objective： To investigate the effects of long non-coding RNA SPRY4-IT1（ Lnc RNA） on proliferation and metastasis of medulloblastoma cells. Methods： SPRY4-IT1 si RNA and control fluorescence si RNA were transfected into medulloblastoma cell line Daoy with Lipofectamine 2000 and were divided into control group and si-SPRY4-IT1 group. Relative expression of SPRY4-IT1 m RNA were detected by real-time quantitative PCR. The change of cell proliferation were examined using CCK-8 kit and clone forming experiment. The change of cell invasion and metastasis were examined by matrigel invasion assay and cell metastasis experiment respectively,The expression of matrix metalloproteinase MMP-2 and MMP-9 were detected by Western blot. Results： In si-SPRY4-IT1 group,the SPRY4-IT1 m RNA expression level,cell proliferation in vitro,cell invasion and migration ability,MMP-2 protein expression were significantly lower than those in the control group. Conclusion： Interference SPRY4-IT1 expression has prominent inhibitory effect on the cell proliferation、invasion and metastasis of medulloblastoma cell line Daoy.
作者
史鹏飞
吉海龙
罗永康
毛天明
陈茜
周开宇
SHI Peng-fei JI Hai-long LUO Yong-kang MAO Tian-ming CHEN Xi ZHOU Kai-yu(the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000 Department of Neurosurgery Department of Pathology, Zhejiang Taizhou Municipal Hospital, Taizhou 318000, China)
出处
《中国应用生理学杂志》
CAS
CSCD
2017年第1期78-82,共5页
Chinese Journal of Applied Physiology
基金
浙江省中医药管理局科技计划资助项目(2013ZA133
2015ZB133)
台州市科技局科技计划资助项目(14SF05)
