摘要
以‘京农8号’红小豆为试验材料,通过盆栽试验方法,采用不同浓度(0、10、20、40和80mg·L^(-1))烯效唑浸种,测定不同水分环境(重度干旱、中度干旱和正常水分)下烯效唑浸种对红小豆生长及根系生理指标,明确干旱环境下红小豆高产优质的最佳烯效唑浸种浓度。结果显示:(1)与正常水分条件相比,干旱胁迫降低了红小豆幼苗叶片叶绿素含量、PSⅡ最大光化学效率(F_v/F_m)、PSⅡ潜在活性(F_v/F_0)和根系抗氧化酶活性、渗透调节物质含量,增加了叶片初始荧光(F_0)、根系MDA含量、根冠比,抑制了红小豆的生长和产量。(2)在不同水分条件下,烯效唑浸种均可有效促进红小豆根系的生长,提高根系SOD活性、POD活性、可溶性糖含量和可溶性蛋白质含量,增强植株抗氧化能力,降低根系MDA的积累量,从而缓解干旱胁迫对质膜的过氧化伤害。(3)烯效唑浸种提高了干旱胁迫下红小豆植株叶片叶绿素含量、F_v/F_m和F_v/F_0,降低了叶片F_0,有效促进干物质的积累,从而有助于红小豆产量构成因素和籽粒产量的提高。研究表明,适宜浓度烯效唑浸种可显著增强红小豆幼苗在干旱胁迫和正常水分环境下光合作用、抗氧化能力和渗透调节能力,有效促进红小豆植株的生长,从而提高了植株的抗旱性和产量,且以20mg·L^(-1)烯效唑浸种处理的效果最好。
The seeds of adzuki bean variety ‘Jingnong No. 8’ were used as experimental material in pot in 2015. The effects of seed soaking with uniconazole on the growth and root physiological characteristics were investigated under different water conditions including normal irrigation, moderate and severe drought, the five different concentrations of uniconazole were 0, 10, 20, 40 and 80 mg·L^-1. The main results showed that： （1） the growth, yield, chlorophyll contents, maximal PSⅡ photochemical efficiency（Fv/Fm）, potential activity of PSⅡ（Fv/F0） and antioxidant enzyme activities of adzuki bean seedling decreased. However, root MDA content and root shoot ratio increased under drought stress. （2） Uniconazole treatment significantly improved the activities of superoxide dismutase, peroxidase, soluble sugar content and soluble protein content, and reduced root MDA content under drought stress （severe and moderate drought） and thus alleviating the oxidative damage of drought stress on plasma membrane. （3） Uniconazole treatment increased chlorophyll contents, Fv/Fm and Fv/F0 while obviously decreased F0 of adzuki bean leaves, which favours the amount of dry matter accumulation, even yield under drought stress. These results showed that uniconazole treatment, especially 20 mg·L^-1 uniconazole, was favorable for the adzuki bean seedlings to increase photosynthetic physiological characteristics and antioxidant enzyme activities, which improved adzuki bean growth and increased drought resistance.
出处
《西北植物学报》
CAS
CSCD
北大核心
2017年第1期144-153,共10页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31571604)
山西省自然科学基金(2013011030-1)
关键词
烯效唑
红小豆
干旱胁迫
生理特性
产量
unieonazole
adzuki bean
drought stress
physiological characteristics
yield
