摘要
背景:Cajal间质细胞病变是糖尿病胃轻瘫的重要发病机制之一,胰岛素、干细胞因子等均可能是Cajal间质细胞病变的上游调控因素。目的:探讨外源性干细胞因子对糖尿病胃轻瘫大鼠胃电及胃阻抗的影响。方法:纳入Wistar大鼠40只,随机分为对照组和观察组,观察组采用一次性腹腔注射链脲佐菌素60 mg/kg并高糖高脂饲料喂食建立糖尿病胃轻瘫大鼠模型,对照组腹腔注射等量生理盐水并正常饮食,8周后检测胃内色素残留率,透射电镜观察胃窦组织超微结构。造模成功后,观察组腹腔注射干细胞因子0.4μg/(kg·d),对照组腹腔注射治疗相同剂量的磷酸盐缓冲液,均连续给予15 d,治疗结束后进行阻抗式胃动力系统检测。结果与结论:(1)观察组胃内色素残留率显著高于对照组(P<0.05);(2)观察组大鼠胃窦Cajal间质细胞间缝隙连接显著减少,呈松散连接状,基膜结构缺乏连续性,细胞内的细胞器数量显著减少,胞质溶解,出现大量的空泡;(3)观察组大鼠治疗后的胃阻抗以及胃电情况均得到显著改善,胃电<2频段功率百分比显著下降,2-4频段功率百分比显著上升(P<0.05);胃阻抗<2频段功率(胃动过缓)百分比显著下降,2-4频段功率百分比显著上升(P<0.05)。治疗后不同频段功率与对照组之间差异无显著性意义(P>0.05);(4)结果表明,糖尿病胃轻瘫大鼠胃排空会出现明显的延迟现象,Cajal间质细胞变性可能是胃轻瘫出现的原因。外源性干细胞因子可以有效改善大鼠的胃电和胃阻抗水平。
BACKGROUND： Lesions in Cajal interstitial cells can lead to diabetic gastroparesis, in which, insulin and stem cell factor are likely to act as upstream regulatory factors. OBJECTIVE： To investigate the effect of exogenous stem cell factors on the gastric electrical activity and gastric impedance in a mouse model of diabetic gastroparesis. METHODS： Forty Wistar rats were randomly divided into two groups, 20 rats in each group. The diabetic gastroparesis model was prepared in observation group by a single use of 60 mg/kg streptozotocin via intraperitoneal injection and high-sugar/high-fat diet. Rats in control group were given the equal volume of norma saline and normal diet. Eight weeks later, residual rate of gastric pigment was detected, and gastric antrum microstructures were observed under transmission electron microscope. If the animal model was successfully prepared, rats were intraperitoneally given 0.4 pg/kg/d stem cell factor in the observation group and same volume of PBS in the control group, for consecutive 15 days. Afterwards, the impedance gastric motility system was detected. RESULTS AND CONCLUSION： Compared with the control group, the residual rate of gastric pigment was significantly higher in the observation group （P 〈 0.05）. In the observation group, there was a certain change in the Cajal interstitial cells in the antrum of stomach, such as the gap junction between the cells were significantly reduced and became loose; there was a lack of continuity of the basal lamina; the number of organelles in the cells was significantly decreased, the cytoplasm was dissolved, and a large number of vacuoles appeared. After treatment with exogenous stem cell factor, the gastric electrical activity and gastric impedance of rats were significantly improved： for the gastric electrical activity, the percentage of 〈 2 band power was significantly decreased, and the percentage of 2-4 band power increased significantly （P 〈 0.05）; for the gastric impedance, the percentage of 〈 2 band power was significantly decreased, and the percentage of 2-4 band power increased significantly （P 〈 0.05）. After treatment, no significant difference in the gastric electrical activity and gastric impedance was found between the two groups. The experimental results show that delayed gastric emptying may occur in diabetic gastroparesis rats, and the degeneration of Cajal interstitial cells may induce gastroparesis. Exogenous stem cells can effectively improve the level of gastric electrical activity and gastric impedance, and thereby improve the .clastric motility in rats.
出处
《中国组织工程研究》
CAS
北大核心
2017年第1期128-132,共5页
Chinese Journal of Tissue Engineering Research
关键词
干细胞
干细胞因子
糖尿病
CAJAL间质细胞
胃轻瘫
,Stem Cell Factor
Interstitial Cells of Cajal
Diabetes Mellitus
Gastroparesis
Tissue Engineering
