摘要
背景:近来有报道指出,维生素C可诱导、加速骨髓间充质干细胞向成骨细胞分化,促进骨的修复和再生,但维生素C水溶液不稳定,因此在其使用时需要载体材料的保护。目的:将磷酸钙作为维生素C的载体,观察其对维生素C的包载及控释能力。方法:采用化学沉淀法制备负载维生素C的磷酸钙颗粒,使维生素C终浓度分别为0,0.1,2,4 mmol/L,检测颗粒载药量。将负载维生素C的磷酸钙颗粒置于模拟体液中,检测维生素C释放量;同时检测超声环境下的维生素C释放量。将磷酸钙颗粒、负载2 mmol/L维生素C的磷酸钙颗粒与MC3T3-E1细胞共培养,1,3,5,7 d后检测细胞增殖,1,5,10,15 d后检测细胞碱性磷酸酶活性。结果与结论:1负载0.1,2,4 mmol/L维生素C磷酸钙颗粒的载药量分别为(59.9±5.4)%、(87.2±1.2)%及(28.4±26.3)%;2在正常环境下,负载0.1,2 mmol/L维生素C的磷酸钙颗粒前期释放速率较慢,负载4 mmol/L维生素C的磷酸钙颗粒前期释放速率较快,3种样品都有持续释药的特点;3在超声环境下,负载2 mmol/L维生素C的磷酸钙颗粒存在一定的突释现象,第1次释放量为5%-15%,而随后的释放速度较缓慢;在75,105和150 W超声辅助条件下,分别持续释放了220,340,260 min;4负载2 mmol/L维生素C的磷酸钙颗粒对MC3T3-E1细胞的增殖无影响,但提高了细胞的碱性磷酸酶活性;5结果表明,磷酸钙可作为维生素C的载体。
BACKGROUND： It is reported that vitamin C can induce bone marrow mesenchymal stem cells differentiating into osteoblasts, and promote bone repair and regeneration. However, vitamin C solution is unstable, so a carrier is necessary.OBJECTIVE： To observe the loading and controlled-release abilities of calcium phosphate used as the carrier of vitamin C. METHODS： Calcium phosphate particles loaded with vitamin C were fabricated using chemical precipitation method, and the final concentration of vitamin C was 0, 0.1, 2 and 4 mmol/L, respectively. The drug-loaded capacity was detected. The release of vitamin from calcium phosphate nanoparticles in the simulate body fluid and ultrasonic environment was respectively evaluated. MC3T3-E1 cells were co-cultured with calcium phosphate nanoparticles loaded with 2 mmol/L vitamin C, or calcium phosphate nanoparticles only. The cell proliferation was detected at 1, 3, 5 and 7 days of culture, and the alkaline phasphatase activity was detected at 1, 5, 10 and 15 days of culture. RESULTS AND CONCLUSION： The drug-loaded contents of calcium phosphate nanoparticles loading 0, 0.1, 2 and 4 mmol/L vitamin C were（59.9±5.4）%,（87.2±1.2）% and（28.4±26.3）%, respectively. Under normal environment, all samples could release vitamin C persistently, but the initial release speed of the particles carrying 0.1 and 2 mmol/L vitamin C was lower than that of particles carrying 4 mmd/L vitamin. Under ultrasonic environment, 2 mmol/L vitamin C-loaded calcium phosphate particles exhibited a quick release speed firstly that reached 5-15%, followed by a slow release speed. When ultrasonic powers kept at 75, 105 and 150 W, the release duration of vitamin C was 220, 340 and 260 minutes, respectively. MC3T3-E1 cell proliferation did not change after co-cultured with 2 mmol/L vitamin C-loaded calcium phosphate particles but the alkaline phosphatase activity was improved. These results suggest that calcium phosphate particles can be used as the carrier of vitamin C.
出处
《中国组织工程研究》
CAS
北大核心
2017年第2期273-279,共7页
Chinese Journal of Tissue Engineering Research
