摘要
流动注射化学发光技术是一种灵敏度高、线性范围宽、分析速度快、重现性好的痕量分析技术。文章研究表明芦丁对高锰酸钾-亚硫酸钠体系发光反应具有较强的催化作用,据此建立了流动注射化学发光测定芦丁的新方法。对影响该体系化学发光强度的诸多因素进行了探讨,在最佳实验条件下,芦丁浓度在5.0×10^(-6)~1.0×10^(-3) mol/L范围内与化学发光强度有良好的线性关系,检出限(3σ)为6.0×10^(-7) mol/L。对5.0×10^(-5)mol/L芦丁标准溶液进行13次平行测定,其相对标准偏差为2.7%。该方法成功地用于药物中芦丁含量的测定。
As a kind of high sensitivity analysis technology, flow injection chemiluminescence possesses a lot of advantages, such as wide linear range, fast analysis speed, good reproducibility. In the paper, a new method for the determination of rutin was developed based on the catalytic action of rutin to thc chemiluminescence reaction of KMnO4 and Na：SO3. Under the opfunal conditions, the linear range of this method to determine rutin was found to be 5.0× 10^-6-1.0× 10^-3 mol/L with a detection limit of 6.0× 10^-7 mol/L and the relative standard deviation was 2.7 % for 13 measurements of 5.0× 10^-5 mol/L rutin standard solution. The method has been successfully used for the determination ofrutin content in drug and food.
出处
《广东化工》
CAS
2017年第2期110-111,共2页
Guangdong Chemical Industry
