摘要
目的:利用HPLC同时测定白花蛇舌草注射液中5种成分的含量。这5种成分包括环烯醚萜苷类(10-乙酰鸡矢藤苷、鸡矢藤苷甲酯、去乙酰车叶草苷酸甲酯)和黄酮苷类{槲皮素-3-O-[2-O-(6-O-E-阿魏酰基)-D-吡喃葡糖基]-β-D-吡喃半乳糖苷、山柰酚-3-O-[2-O-(6-O-E-阿魏酰基)-β-D-吡喃葡糖基]-β-D-吡喃半乳糖苷}。方法:采用Sun FireTMC18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水(含0.1%甲酸)为流动相,梯度洗脱,柱温30℃,流速1.0 m L·min-1。结果:各对照品在线性范围内线性关系良好,相关系数均≥0.999 5;加样回收率为98.1%~101.2%,RSD均≤3.3%。结论:该方法操作简便,结果可靠,重复性好,可作为白花蛇舌草注射液质量评价的参考依据。
Objective： To develop an HPLC method for simultaneous determination of five compounds,including three iridoid glucosides（ 10-acetyl-scandoside,scandoside methyl ester,and deacetylasperuloside methyl ester） and two flavonoid glucosides { quercetin-3-O- [2-O-（ 6-O-E-ferloyl）-β-D-glucopyranosyl ]-β-Dgalactopyranoside and kaempferol-3-O- [2-O-（ 6-O-E-ferloyl）-β-D-glucopyranosyl]-β-D-galactopyranoside} in Hedyotis diffusa injection. Method： The separation of targeted compounds was performed on Sun Fire^TMC18column（ 4. 6 mm × 250 mm,5 μm） with acetonitrile-0. 1% formic acid solution as the mobile phase for gradient elution.The column temperature was 30 ℃ and the flow rate was 1. 0 m L·min^-1. Result： The method showed good linearity（ r〉 0. 999 5） within the linear range. The recoveries were in the range of 98. 1%-101. 2% with RSD 〈3. 3%. Conclusion： The method is reliable,simple and reproducible,and it can be used to control the quality of Hedyotis diffusa injection.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第4期89-92,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省自然科学基金项目(B2013208018)
