摘要
目的通过Box-Behnken响应面法优化川芎Ligusticum chuanxiong Hort.水提液纳滤工艺。方法在单因素试验基础上,以纳滤膜截留分子量、提取液质量浓度、p H值为影响因素,阿魏酸截留率为评价指标,Box-Behnken响应面法优化工艺。结果最佳条件为纳滤膜截留分子量100 Da,提取液质量浓度20.58μg/m L,p H值7.69,阿魏酸截留率达93.00%,与实测值(91.23%)接近。结论该方法合理可行,可用于纳滤川芎水提液。
AIM To optimize the nanofiltration of Ligusticum chuanxiong Hort. aqueous extract by Box-Behnken response surface method. METHODS In addition to the influencing factors of molecular weight cut-off of nanofiltration membrane,extract concentration and p H value,the evaluation index of fumalic acid retention rate was taken into account for the process optimization by Box-Behnken response surface method on the basis of single factor test. RESULTS The optimal conditions were determined to be 100 Da for molecular weight cut-off of nanofiltration membrane,20. 58 μg / m L for extract concentration,and 7. 69 for p H value. The retention rate of fumalic acid reached 93. 00%,approximating the measured value（ 91. 23%）. CONCLUSION This reasonable and feasible method can be used for the nanofiltration of L. chuanxiong aqueous extract.
出处
《中成药》
CAS
CSCD
北大核心
2017年第2期296-300,共5页
Chinese Traditional Patent Medicine
基金
国家自然科学基金(81503258
81373980)
江苏省自然科学基金青年基金(BK20151005)
江苏省中医药局科技项目(YB2015009)
关键词
川芎
水提液
纳滤
Box-Behnken响应面法
Ligusticum chuanxiong Hort.
aqueous extract
fumalic acid
nanofiltration
Box-Behnken re sponse surface method
