摘要
目的探讨葛根饮料对小鼠酒精性肝损伤的保护作用。方法将实验小鼠随机分为模型组、空白对照组、阳性对照组、高浓度、中浓度、低浓度组6组,分别用体积分数50%的乙醇灌胃,造成小鼠酒精性肝损伤,灌胃量为12m L/kg·BW,同时,除模型组外,高、中、低三浓度组分别灌胃实验室配制的1/2倍、1倍和2倍浓度的葛根饮料,阳性对照组灌胃RU-21药,空白组灌胃生理盐水,每天灌胃2次,每次间隔12h。实验四周后,取小鼠肝脏组织,测定各组小鼠肝匀浆丙二醛(MDA)浓度、谷胱甘肽过氧化物酶(GSH-Px)、甘油三酯(TG)含量和血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)活力,评估葛根饮料对小鼠酒精性肝损伤的治疗作用。结果与模型组相比,3个浓度组小鼠肝匀浆GSH-Px含量明显升高(P<0.01),TG含量明显下降(P<0.05),高浓度组、阳性对照组小鼠血清ALT含量下降(P<0.05)。结论葛根饮料对小鼠酒精性肝损伤具有一定的肝脏保护作用。
OBJECTIVE The purpose of the present study was to investigate the effect of pueraria lobata beverage on alcohol-induced liver injury in mice. METHODS Divided mice into 6 groups,each group was intragastric administration with 50% ethanol（ 12 m L·kg-1）. At the same time,high,medium and low concentration groups were lavaged 1 /2,1 and 2 times content of puerarin beverages,positive group was lavaged RU-21 medicine,blank group was lavaged saline. They were lavaged 2 times per day. After experiment,the technique was performed to measure the malondialdehyde（ MDA） concentration,glutathione peroxidase（ GSH-Px）,triglycerides（ TG） levels and serum aspertate aminotransferase（ AST）,as well as alanine aminotransferase（ ALT）. RESULTS The results showed that,compared with alcohol-induced group,pretreatment lavaged with low,normal,high content pueraria lobata beverage groups,homogenate gsh-px levels increased significantly（ P〈0. 01）,TG content significantly decreased（ P〈0. 05）,high concentration group,positive control group mice serum ALT levels decreased（ P〈0. 05）. CONCLUSION Pueraria lobata beverage can protect alcoholic liver injury.
出处
《海峡药学》
2017年第1期28-31,共4页
Strait Pharmaceutical Journal
关键词
葛根饮料
酒精性肝损伤
小鼠
Puerarin beverages
Alcoholic liver injury
Mice
