摘要
深入理解小麦氮利用效率基因型差异的分子生物学机理对于氮高效小麦的分子育种具有重要指导意义。本试验选用两个不同氮利用效率的小麦基因型,设置不同氮水平,分别在小麦的5个生育期收获采样,研究不同氮利用效率小麦基因型中5个氮代谢相关基因的表达特征。研究结果表明,在氮利用方面,无论是在低氮还是高氮条件下,氮利用高效小麦XY107的籽粒氮利用率均高于氮利用低效小麦LM1;在基因表达方面,在低氮条件下,小麦地上部谷氨酰胺合成酶基因(TaGS1c)、丙氨酸转氨酶基因(TaAlaAT)和丙酮酸磷酸双激酶基因(TaPPDK)的表达水平在抽穗期以后均显著增强,且在氮高效小麦XY107中的表达水平显著高于在氮低效小麦LM1中的表达水平;而在高氮条件下,只有基因TaPPDK的表达水平在抽穗期以后显著增强,且在氮高效小麦XY107中的表达水平显著高于在氮低效小麦LM1中的表达水平。本研究发现,TaGS1c、TaAlaAT和TaPPDK 3个基因在决定小麦氮利用效率的基因型差异方面发挥着重要作用。
Understanding the molecular mechanism of the genotypic differences in nitrogen use efficiency of wheat is very important for the molecular breeding of N-efficient wheat varieties. Seasonal expression patterns of five genes（ TaGS1 c,TaGS2 a,TaAla AT,TaASN1 and TaPPDK） involved in nitrogen（ N） metabolism in two wheat genotypes with high and low nitrogen utilization efficiencies of winter wheat were investigated. Shoots were sampled at the tillering,jointing,heading,grain filling and harvest stages. The results showed that the grain nitrogen utilization efficiencies of wheat genotype XY107 was greater than that of LM1 in both LN and HN treatments; The expression levels of the three genes TaGS1 c,TaAla AT and TaPPDK in the N-efficient wheat genotype XY107 were significantly greater than that in LM1 after the heading stage in the low N treatments; Only the expression levels of the genes TaPPDK was significantly enhanced after heading stage and in the N-efficient wheat genotype XY107 were significantly greater than that in LM1 in the high N treatments. The present study suggested that the genes TaGS1 c,TaAla AT and TaPPDK might play important roles in determining the genotypic differences of the nitrogen use efficiency of wheat.
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2017年第1期48-55,共8页
Soil and Fertilizer Sciences in China
关键词
小麦
氮利用率
基因表达
wheat
nitrogen utilization efficiency
gene expression
