摘要
利用壳聚糖絮凝纯化作用精制蹄叶橐吾叶多糖,以多糖损失率,蛋白质去除率及脱色率为指标,通过单因素及正交试验确定最佳工艺参数:在50 m L粗多糖溶液(浓度为20 mg/m L)中加入2%壳聚糖溶液1 m L,絮凝温度30℃下处理60 min,得到的精制蹄叶橐吾叶多糖(DPLF)多糖损失率为42.81%,蛋白质脱除率为85.03%,脱色率为81.74%。DPLF抗氧化活性结果表明:DPLF总抗氧化能力和还原力均随浓度增加呈上升趋势,清除DPPH自由基能力、清除超氧阴离子能力和清除羟基自由基能力的IC_(50)值分别为0.035 1、1.459 1 mg/m L和0.154 8 mg/m L。
The polysaccharide of Ligularia fischeri（PLF）were purified using chitosan method. Indicates for polysaccharide loss rate,removal rate of protein and decolorization rate,the optimum parameter were determined that chitosan solution（concentration of 2 %）of 1 m L was added,and pured 60 min under the temperature of 30 ℃ in PLF solution of 50 m L（concentration of 20 mg/m L）to obtain the DPLF. Polysaccharide loss rate was 42.81 %,removal rate of protein was 85.03 % and decolorization rate was 81.74 %.Antioxide activity of DPLF showed that total antioxide activity and reducing power appeared the trend of rising with concentration increasing. IC_（50） values of scavenging activity of DPLF on DPPH free radicals,superoxide anion radicals and hydroxyl free radicals is 0.035 1,1.459 1 mg/m L and 0.154 8 mg/m L,respectively.
出处
《食品研究与开发》
CAS
北大核心
2016年第19期25-29,共5页
Food Research and Development
关键词
蹄叶橐吾叶多糖
壳聚糖法
絮凝作用
抗氧化活性
polysaccharide of Ligularia fischeri
chitosan method
flocculation
antioxide activity
