摘要
应用紫外分光光度计和氨基酸自动分析仪检测胶原蛋白水解物中L-羟脯氨酸含量,分析了两种方法的适用性。结果显示,紫外分光光度计法和氨基酸分析仪法在0～2.95 mg/L范围内,L-羟脯氨酸的浓度分别和吸光度、峰面积的线性良好,相关系数R2分别为0.999 3和0.999 8,RSD分别为0.96%和0.73%（n=6）。紫外分光光度计法样品处理简便且重复性好,适合基层大量检测使用;氨基酸分析仪法操作简便且不受其他氨基酸干扰,适合精密和研究检测使用。
The content of L-Hydroxyproline（Hyp）in collagen hydrolysate was measured by An UV spectrophotometer and an amino acid auto-analyzer（AAA）respectively. And the applicability of the two methods was demonstrated. The results showed that the linear relationships between L-Hydroxyproline concentration and absorbance,peak area were both good in the range of 0-2.95 mg/L. R2 were 0.999 3 and 0.999 8 respectively.And RSD were 0.96 % and 0.73 %（n=6）respectively. The UV Spectrophotometry was suitable for basic level detection because the samples could be handled easily and had a good reproducibility. The automatic amino acid analyzer was suitable for precision and research because of the advantages of easy operation and not affected by other amino acids.
出处
《食品研究与开发》
CAS
北大核心
2016年第22期119-122,共4页
Food Research and Development
基金
国家高技术研究发展计划(863计划)项目(2013AA102205-02)
科技北京百名领军人才培养工程项目(Z131110000513026)
