摘要
建立Qu ECh ERS-液相色谱串联质谱法同时检测禽肉中的丹诺沙星、恩诺沙星、沙拉沙星、环丙沙星、氧氟沙星、诺氟沙星、依诺沙星、培氧沙星、双氟沙星9种喹诺酮类兽药残留量方法。样品以乙腈、EDTA-Mcllvaine和乙酸为提取试剂,无水硫酸钠为脱水剂,C18为吸附剂进行净化,以甲醇-0.1%甲酸水为流动相,反相C18色谱柱分离,正离子多反应监测模式进行质谱分析。9种喹诺酮在1.0μg/kg^200μg/kg范围内具有良好的线性关系,相关系数(r2)均在0.993以上。检出限范围为0.07μg/kg^0.20μg/kg,定量限范围在0.23μg/kg^0.67μg/kg之间。平均回收率介于70.8%~93.0%之间,相对标准偏(RSD)低于8.9%。该方法适合于检测禽肉中的喹诺酮类药物残留量的测定。
A method was developed for simultaneous determination of 9-quinolones（danofloxacinmesylate,enrofloxacin, sarafloxacin,ciprofloxacin,ofloxacin,norfloxacin,enoxacin, pefloxacin, difloxacin） in poultry by Qu ECh ERS-high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）. The chicken muscle was extracted with acetonitrile,EDTA-Mcllvaine and acetic acid,dehydrated with Na2SO4,purified with C18 sorbent and separated by C18 column using methanol and water（containing 0.1 % formic acid）as mobile phase. The mass spectrometric acquisitions were carried out by means of multiple reaction monitoring（MRS）in positive ionization mode. The linear relation of 9 quinolones was good and the correlation coefficient（r2）was more than 0.993. The limit of detection（LOD）of 9 quinolones was 0.07 μg/kg-0.20 μg/kg and the limit of quantification（LOQ）was 0.23 μg/kg-0.67 μg/kg. The recovery rates of the target analyte were between 70.8 %-93.0 %. The relative standard deviations（RSD）were less than 8.9 %. The method was reliable for the determination of quinolones inpoultry.
出处
《食品研究与开发》
CAS
北大核心
2016年第22期153-157,共5页
Food Research and Development
