摘要
目的观察电针对2型糖尿病合并肾性高血压大鼠模型的疗效,并探索其可能的作用机制,进一步完善针灸在临床急、慢性疾病中的应用。方法将50只Wistar大鼠随机选择10只作为空白组,余下40只用于模型制作,并随机均分为单纯糖尿病组、单纯肾性高血压组、复合模型电针组、复合模型未电针组。大鼠高脂高糖膳食4周后,予快速腹腔注射链脲佐菌素(STZ),制作成单纯2型糖尿病模型,待模型血糖稳定,再用改良的"两肾一夹法"结扎大鼠单侧肾动脉,造成肾动脉狭窄,形成肾性高血压。最后获得稳定的2型糖尿病合并肾性高血压病的复合模型。其中除复合模型未电针组大鼠不予电针治疗外,其余各组大鼠均给予两个周期(2周)的电针治疗。最后,检测各组大鼠电针前后的血压(BP)、空腹血糖(FBG)、血肌酐(Cr)、尿素氮(BUN)、糖化血红蛋白(GHb A1c)、肾素(PRA)及血管紧张素II(Ang II)值的变化。结果电针2周后,复合模型电针组与复合模型未电针组相比,BP、FBG、GHb A1c、PRA、Ang II值均降低(P<0.01),但Cr和BUN值变化不明显(P>0.05)。结论电针刺激2型糖尿病合并肾性高血压模型大鼠的双侧足三里穴,能使复合模型大鼠的血糖和血压降低至正常水平,且经单次电针刺激后2-3天内,复合模型大鼠的血糖和血压均能稳定在治疗后水平。另外,针灸疗法较其他治疗方法还具有经济、安全、无副作用等优点,更适合应用于2型糖尿病合并肾性高血压病的早期临床治疗及基础研究。
Objective To observe the therapeutic effect of acupuncture therapy for the rat model of type 2 diabetes mellitus（ T2DM） combined with renal hypertension and try to explore its mechanism. Methods We randomly select 10 Wistar rats as the blank group and 40 rats were used to make the model groups,which were divided into simple diabetes group,simple renal hypertension group,the compound group with electroacupuncture and the compound group without electroacupuncture,with 10 rats in each group. After a high fat and sugar diet for 4 weeks,the Wistar rats were given streptozotocin i. p. injection to establish models of type 2 diabetes mellitus. Renal hypertension was developed by the＂2K1C＂improved method to make unilateral renal artery ligation-induced renal artery stenosis. Then,electroacupuncture treatment was performed on the rats for 2 weeks except the compound group without electroacupuncture. The changes of values of BP,FBG,Cr,BUN,glycated hemoglobin,renin and Ang II were recorded and analyzed. Results The values of BP,FBG,Cr,BUN,glycated hemoglobin,renin and Ang II in the compound group with electroacupuncture showed a significant reduction compared with the compound group without electroacupuncture after 2 weeks（ P〈0. 01）,but there was no obvious changes in the values of Cr and BUN（ P〈0. 05）. Conclusions The blood glucose and blood pressure in the rat model of compound group can be reduced to a normal level with continuous electroacupuncture at bilateral acupoints Zusanli,and it can also be kept at a stable level after single electroacupuncture for 2- 3 days. The acupuncture therapy is more suitable for early clinical treatment and can be used in basic research with advantages of economic,safe,no side effect and so on.
作者
万斌
孙丽薇
刘蓉
李洪涛
顾为望
WAN Bin SUN Li-wei LIU Rong LI Hong-tao GU Wei-wang(The First Affiliated Hosptital of Guangzhou Medical University,Guangzhou Institute of Respiratory Diseases State Key Laboratory of Respiratory Diseases,Guangzhou 510120,China Department of Medical Genetics, Zhongshan Medical College of Sun Yat-sen University,Guangzhou 510080 Laboratory Animal Center, Southern Medical University, Guangzhou 510515)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2016年第6期622-627,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
广东省科技基础条件建设项目(编号:2014A030304069
2015A030303016)
关键词
电针
糖尿病
肾性高血压病
足三里
大鼠
Electroacupuncture
Type 2 diabetes mellitus
Renal hypertension
Acupoint ST36（Zusanli）
Rat
