摘要
为了研发针对地下害虫蛴螬的Bt新型制剂以提高其防治效果,本研究成功构建了能够表达绿色荧光蛋白的菌株Bt185-gfp,筛选出了对Bt增殖效果优良的花生粕用于防治地下害虫专用剂型的开发。花生粕在1%(质量与体积比)情况下与实验室常用LB培养基相比对Bt增殖无显著差异,说明花生粕是一种营养丰富、适合Bt增殖的材料。花生粕加入Bt菌粉压制成颗粒后,在实验室无菌条件下,颗粒剂中Bt菌体最高增殖了30倍左右;而在土壤环境中,菌体在颗粒剂内增殖了40倍左右。生物测定结果显示,在初始Bt菌量相同情况下,颗粒剂的杀虫活性由未加入花生粕的19.44%提高到加入后的61.11%,杀虫效果增强显著。本研究发现膨化处理不会影响Bt增殖效果,且与灭菌的花生粕获得的Bt活菌数量无显著差异。此外,还评估了利用花生粕增殖其他几种生防菌株的能力,结果发现花生粕能够有效增殖这几种菌株。本研究为防治地下害虫和土传病害生物农药新剂型的开发提供了新思路。
To develop a new Bt formulation and improve its control efficiency against soil pest grub, the Bt185-gfp strain expressing the green fluorescent protein was constructed, and a special formulation for control of soil grub pest was developed using the peanut-meal that showed excellent multiplication for the Bt strain. There was no significant difference in Bt multiplication between 1% peanut-meal （W/V） and LB medium, indicating that the peanut-meal was a nutrient-rich and suitable material for Bt multiplication. When the peanut-meal and Bt spore powder were mixed together and suppressed into granules, the Bt in the granules multiplied by 30 times under laboratory sterile condition and by 40 times in soil. The granules caused a 61.11% mortality of the pest, which was much higher than the 19.44% mortality resulted from granules without peanut-meal, even when the two kinds of granules were of the same initial amount of Bt. We also found that the expanding process did not affect Bt multiplication, and produced Bt population size not different from that obtained from the sterilization process. In addition, the peanut-meal was also suitable for multiplication of other bio-control strains. This study provides a new way to develop a new formulation for soil pest and disease control.
出处
《中国生物防治学报》
CSCD
北大核心
2017年第1期70-78,共9页
Chinese Journal of Biological Control
基金
国家自然科学基金(31301731
31428020)
关键词
地下害虫
蛴螬
BT
增殖
underground pest
white grub
Bt
multiplication
