摘要
目的:探讨新疆地区核包膜蛋白抗体(GP210)、核多点抗体(SP100)及抗线粒体抗体M2亚型(AMA-M2)检测对原发性胆汁性肝硬化(PBC)的临床意义.方法:选取126例PBC患者与585例非PBC患者,免疫印迹法检测GP210、sp100及AMA-M2并分析其诊断PBC的临床评价指标.结果:GP210和SP100的诊断灵敏性与阴性预测值均显著低于AMA-M2,SP100阳性预测值显著低于GP210与AMA-M2,差异有统计学意义(P<0.05).GP210与SP100在维族PBC患者中的阳性率显著高于汉族,差异有统计学意义(P<0.05);AMA-M2检测阳性率在维、汉族PBC患者中高达90.6%与93.5%,差异无统计学意义(P>0.05).AMA-M2阴性PBC患者GP210与sp100抗体阳性率分别为60.0%和50.0%.结论:AMA-M2对PBC的诊断效能较高并且不存在维、汉族差异,但GP210与SP100对于AMA-M2阴性PBC患者具有补充诊断意义.
AIM： To investigate the clinical significance of nuclear envelope protein antibody（ Gp210）,Nuclear multiple point antibody（ sp100） and M2 subtype anti mitochondrial antibody（ AMA-M2） detection for primary biliary cirrhosis（ PBC） in Xinjiang Uygur Autonomous Region. METHODS： A total of 126 cases of PBC and 585 cases of non PBC were selected as the objects of study. The Gp210,sp100 and AMAAMA-M2 were detected by immunoblotting,and the clinical value of them for PBC were analyzed.RESULTS： In PBC diagnosis,sensitivity and negative predictive value of GP210 and sp100 were both significantly lower than AMAM2,and the positive prediction value of sp100 was significantly lower than GP210 and AMA-M2,and the difference was statistically significant（ P〈0.05）. Positive rate of GP210 and sp100 in the Uygurs were significantly higher than those of Han nationality,and the difference was statistically significant（ P〈0.05）. Positive rate of AMA-M2 in the Uygurs and Han nationality respectively were 90. 6% and93.5%（ P〉0.05）. Positive rate of GP210 and SP100 in the AMA- M2 negative PBC cases are 60. 0% and 50. 0%. CONCLUSION： AMA-M2 for PBC has much higher diagnosis efficiency,and no significant difference was found between the Uygurs and the Han nationality. However,GP210 and sp100 are necessary additional diagnosis for AMA-M2 negative PBC patients.
出处
《转化医学电子杂志》
2017年第2期48-49,共2页
E-Journal of Translational Medicine
