摘要
该文建立了大孔树脂-高速逆流色谱分离薇甘菊中黄酮类物质的方法。分离条件为:采用大孔树脂AB-8,洗脱液为50%(v/v)乙醇水溶液,高速逆流色谱溶剂体系为正丁醇-乙酸-水(4∶1∶5,v/v)。从薇甘菊中分离到4种黄酮类物质:槲皮素-3-O-芸香糖苷(纯度90.2%)、山奈酚-3-O-芸香糖苷(纯度98.55%)、木犀草苷(纯度98.33%)和紫云英苷(纯度99.23%)。建立的大孔树脂-高速逆流色谱方法简单、高效,可扩展应用于从其他植物中分离黄酮类物质。
The present study established a method for the separation and purification of four flavonoids quercetin-3-O- rutinoside, kaempferol-3-O- rutinoside, luteoloside and astragalin from Mikania micrantha by using macroporous resin combined with high speed countercurrent chromatography （HSCCC）. Macroporous resin was AB-8 and the eluent was 50% （v/v） ethanol. A two-phase solvent system composed of nbutanol/acetic acid/water （4：1：5, v/v） was used in HSCCC. Quercetin-3-O- rutinoside, kaempferol-3-O- rutinoside, luteoloside and astragalin with the purity of 90. 12%, 98. 55%, 98. 33% and 99. 23%, respectively, were successfully separa-ted from Mikania micrantha by HSCCC using the lower phase of this solvent system as mobile phase in one run. This study pave a simple and efficient method that could be used in the following separation of flavonoids from invasive plants.
出处
《色谱》
CAS
CSCD
北大核心
2017年第3期302-307,共6页
Chinese Journal of Chromatography
基金
National Natural Science Foundation of China(No.21075007)
Program for New Century Excellent Talents in University(No.NCET-11-0563)
Beijing Nova Program Interdisciplinary Cooperation Project(No.Z161100004916045)
关键词
高速逆流色谱
大孔树脂
分离
黄酮类物质
薇甘菊
high speed countercurrent chromatography （HSCCC）
macroporous resin
separation
fla-vonoids
Mikania micrantha （M. micrantha）
