摘要
研究螺旋藻多糖和小球藻多糖复合后的抑菌及抗氧化效果。采用滤纸片法研究复合多糖的抑菌效果,并测定复合多糖对DPPH自由基、超氧阴离子自由基等的抗氧化能力。结果表明,当小球藻多糖与螺旋藻多糖混合比为1∶4时,对大肠杆菌抑菌效果最好;各比例多糖对藤黄八叠球菌的抑制作用不明显,当该混合比为1∶6时对假丝酵母达到最好抑菌效果。在抗氧化试验中该复合多糖混合比1∶2时,清除DPPH自由基表现的最好;混合比1∶3时其还原力的表现优于其他组。而当螺旋藻多糖与小球藻多糖混合比1∶2时的抗脂质过氧化能力最好,清除羟基自由基效果也最好;当复合多糖混合比1∶3时,清除超氧阴离子效果最好。以上结果说明复合多糖的抗氧化效果要优于单一多糖,可作为新型天然抗氧化剂进行后续开发利用。
The effects of the mixture of Spirulina polysaccharide and Chlorella polysaccharide on antimicrobial and antioxidant bioactivities were studied. Filter paper method was used for detection of antibacterial bioactivi-ties of the polysaccharides compounds,and antioxidant activities of the polysaccharides compounds were assayed by scavenging DPPH free radical and superoxide anionree free radica,etc. The result showed that the polysaccharides compounds had good activities in inhibiting the growth of the strain of Escherichia coli but not in Sarcina lutea when the mixture ratio of Chlorella polysaccharide and Spirulina polysaccharide was 1 ∶ 4. The polysaccharides compounds showed effective inhibitory action against the selected pathogen Candida Albicans when the mixture ratio was 1 ∶ 6. The antioxidant activities polysaccharides compounds were he best effects ofDPPH radical-scavenging when the mixture ratio was 1 ∶ 2. Reducing power of the polysaccharides compounds performed better than the other treatment groups when the mixture ratio was 1 ∶ 3. Moreover,the polysaccharides compounds possessed good scavenging activity of lipid peroxidation and hydroxyl free radical-scavenging activity when the mixture ratio of Spirulina polysaccharide and Chlorella polysaccharide was 1 ∶ 2. Also,the new polysaccharides compounds showed significant superoxide anion-scavenging activity when the mixture ratio was1 ∶ 3. Above all,the polysaccharides compounds possessed higher antioxidant capacities than Spirulina polysaccharide or Chlorella polysaccharide and thus could be potential new sources of natural antioxidants for further development and utilization.
作者
刘凤路
张福
邸富荣
宋东辉
LIU Feng-lu ZHANG Fu DI Fu-rong SONG Dong-hui(College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin 300457, China)
出处
《食品研究与开发》
CAS
北大核心
2017年第2期16-22,共7页
Food Research and Development
基金
天津市海洋局科技兴海项目(KJXH2013-16)
关键词
微藻复合多糖
抑菌效果
抗氧化能力
microalgae polysaccharides compounds
antimicrobial activities
antioxidant capacities
