摘要
该实验筛选了芍药苷经鼻给药制剂的吸收促进剂,建立了HPLC测定灌流液中芍药苷的方法,采用改良的大鼠鼻腔灌流模型,筛选吸收促进剂的种类和用量。为了避免鼻腔的分泌和吸收对灌流液体积的影响,采用体积校正法计算剩余药量,各时间点剩余药量的百分率取对数后与对应时间进行回归,回归直线的斜率即为吸收速度常数。结果显示,羟丙基-β-环糊精和水溶性氮酮能够显著提高芍药苷的鼻腔吸收,其中水溶性氮酮吸收速度常数最大,对芍药苷的促渗效果最好。当灌流液中水溶性氮酮的质量浓度由5 g·L^(-1)逐渐升高为20 g·L^(-1)时,吸收速度常数逐渐变大,20 g·L^(-1)时达到峰值;当其质量浓度增加至30 g·L^(-1)时,吸收速度常数变小,表明水溶性氮酮质量浓度为20 g·L^(-1)时促渗效果最好。
This experiment was aimed to screen the absorption enhancer for intranasal administration preparations of paeoniflorin. In this study,HPLC method for determination of paeoniflorin in perfusion liquid was established and the improved model of nasal perfusion in rats was used to screen out the species and amounts of absorption enhancer. In order to avoid the influence of the secretion and absorption of nasal cavity on the volume of perfusion fluid,the residual dose was calculated by using the volume correction method. Linear regression was carried out between the logarithm to the percentage of the residual dose and the corresponding time,and the slope of the regression line was exactly the absorption rate constant. Experimental results showed that hydroxypropyl-β-cyclodextrin and water-soluble azone can significantly improve the nasal absorption of paeoniflorin. Furthermore,water-soluble azone had the highest absorption rate constant and the best promoting penetration effect on intranasal administration preparations of paeoniflorin. It was also found that when the mass concentration of water-soluble azone in the perfusion liquid increased from 5 g· L^（-1）to 20 g· L^（-1）,the absorption rate constant was gradually increased and peaked at 20 g·L^（-1）. When the mass concentration was increased to 30 g· L^（-1）,the absorption rate constant was decreased,indicating that the best mass concentration of water-soluble azone was 20 g· L^（-1）.
作者
朱智涛
潘金火
吴贤慧
蒋志涛
ZHU Zhi-tao PAN Jin-huo WU Xian-hui JIANG Zhi-tao(College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China Graduate Workstation of Enterprises in Jiangsu Province, Zhangjiagang Hospital of Traditional Chinese Medicine Group Corporation, Zhangjiagang 215600, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第3期493-497,共5页
China Journal of Chinese Materia Medica
基金
江苏省科技厅社会发展项目(BE2009682)
关键词
芍药苷
大鼠鼻腔灌流
吸收促进剂
水溶性氮酮
paeoniflorin
nasal perfusion in rats
absorption enhancer
water-soluble azone
