摘要
目的探讨脓毒血症患者血清IL-6以及IL-10的表达及对免疫功能的影响。方法选择2013年6月-2015年6月在我院重症医学科(ICU)收治的50例脓毒血症患者为实验组,2015年1月-2015年6月来我院体检的健康人群50例为对照组,检测两组血清IL-6、IL-10以及T细胞亚群水平;分析IL-6、IL-10水平与CD4+/CD8+比值的相关性。结果实验组患者血清IL-6以及IL-10水平均明显高于对照组,差异均有统计学意义(P<0.05)。与对照组比较,实验组CD3+、CD4+、CD8+及CD4+/CD8+均有不同程度降低,差异有统计学意义(P<0.05)。相关分析结果显示,IL-6、IL-10水平与CD4+/CD8+比值均呈显著负相关(r分别为-0.328,-0.452;P均<0.05)。结论脓毒血症患者血清中IL-6和IL-10水平呈高表达、T细胞亚群呈低表达,且三者间具有协同作用,可以共同促进病变的进展。因此,在早期阶段检测IL-6、IL-10及T淋巴细胞亚群可以作为早期诊断脓毒血症的指标,对于疾病的预后判断也有一定的指导意义。
Objective To investigate the expression of serum IL-6and IL-10 in sepsis patients and influence on immune system.Methods 50 cases of sepsis patients in ICU in our hospital from June 2013-June 2015 were selected as the experimental group,50 healthy people to examination in our hospital from January 2015-June 2015 were selected as control group.The serum IL-6,IL-10 and T cell subsets of the two groups were detected.The correlation between serum IL-6,IL-10 and the ratio of CD4^＋/CD8^＋were analysed.Results The serum IL-6and IL-10 levels of the experimental group were significantly higher than that of the control group,the differences were statistically significant（P〈0.05）.Compared with the control group,CD3^＋,CD4^＋,CD8^＋and CD4^＋/CD8^＋in the experimental group were reduced to varying degrees,the difference was statistically significant（P〈0.05）.The results of correlation analysis showed that IL-6,IL-10 and the ratio of CD4^＋/CD8^＋was negatively correlated（r =-0.328,-0.452;P〈0.05）.Conclusion There were higher expression of IL-10 and IL-6,and lower expression of T cell subsets in serum of patients with sepsis;The effect is congenerousbetween the three common,which promote lesions progress.Therefore,the detection of IL-6,IL-10 and T lymphocyte subsets in the early stages can be an indicator in the diagnosis of sepsis,and help to estimate the prognosis.
出处
《中国实验诊断学》
2017年第2期279-281,共3页
Chinese Journal of Laboratory Diagnosis
