摘要
目的探讨EV71脑炎患儿血清和脑脊液(cerebrospinal fluid,CSF)中血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)和血管细胞粘附分子-1(vascular cell adhesion molecule-1,VCAM-1)水平的变化及其临床意义。方法根据临床表现将患儿分为普通病例组、重症病例组(再分为重型组和危重型组)。并设立对照组。应用双抗体夹心ELISA法检测各组患儿血清和CSF中VEGF和可溶性血管细胞粘附分子-1(soluble vascular cell adhesion molecule-1,s VCAM-1)的水平。结果普通病例组血清和CSF中VEGF、s VCAM-1水平与对照组差异无统计学意义(均P>0.05)。重症病例组血清和CSF中VEGF、s VCAM-1水平均显著高于普通病例组和对照组,差异有统计学意义(均P<0.01)。危重型组血清和CSF中VEGF、s VCAM-1水平均显著高于重型组,差异有统计学意义(P<0.01或P<0.05);且二组血清和CSF中VEGF、s VCAM-1水平均显著高于对照组,差异有统计学意义(P<0.01或P<0.05)。重症病例组急性期血清和CSF中VEGF、s VCAM-1含量均显著高于恢复期和对照组,差异有统计学意义(均P<0.01)。重症病例组血清与CSF中VEGF、s VCAM-1均呈正相关(r=0.587、0.648,均P<0.01);普通病例组、对照组血清与CSF中VEGF、s VCAM-1均无相关性。结论 VEGF、VCAM-1可能参与EV71脑炎的发病机制,对EV71脑炎的病情严重性评估及预后判断有一定意义。
Objective To explore the changes of vascular endothelial growth factor（VEGF）and vascular cell adhesion molecule-1（VCAM-1）in serum and cerebrospinal fluid（CSF） and their clinical values in children with EV71 encephalitis. Methods According to the clinical manifestations, children patients were divided into ordinary case group and severe case group （which were subdivided into heavy type group and critical type group）. A control group was set. The concentrations of VEGF and sVCAM-1 in serum and CSF of each patient of each group were detected by double antibody sandwich ELISA method. Results The levels of VEGF and sVCAM-1 in serum and CSF were not significantly different between the ordinary case group and control group （both P〉0.05）, but significantly higher in the severe case group than the ordinary case group and control group（all P〈0.01）, in the critical type group than the heavy type group（P〈0.01 or P〈0.05）, in the critical type group and heavy type group than the control group （P〈0.01 or P〈0.05）, as well as in the acute phase of severe case group than in the recovery phase and than the control group（all P〈0.01）. Serum and CSF VEGF, sVCAM-1 were positively correlated in the severe case group （r=0.587, r=0.648, both P〈0.01） , while there were not significant correlations in the ordinary case group and control group. Conclusions VEGF and VCAM-1 may participate in the pathogenesis of EV71 encephalitis. Detection of the two parameters may be helpful to evaluating the severity and prognosis of EV71 encephalitis.
出处
《实用预防医学》
CAS
2017年第3期287-290,共4页
Practical Preventive Medicine
基金
浙江省医药卫生科技计划项目(2011KYA130)
杭州市科技发展计划项目(20110833B18)
关键词
EV71脑炎
血管内皮细胞生长因子
血管细胞粘附分子-1
儿童
EV71 encephalitis
vascular endothelial growth factor （ VEGF ）
vascular cell adhesion molecule- 1 （ VCAM- 1 ）
children
