摘要
目的采用指纹图谱与一测多评(QAMS)结合的方法评价荔枝多酚提取物,并测定提取物中4种多酚类成分。方法采用RP-UPLC法测定22批荔枝多酚提取物,建立指纹图谱的共有模式。以表儿茶素为内参物,建立原花青素A2、原花青素B2、表儿茶素-(4β→8,2β→O→7)-表儿茶素-(4β→8)-表儿茶素(PC-C)的相对校正因子(f),并测定各成分量,实现QAMS。同时比较QAMS和外标法测定4种多酚类成分量测得值的差异,验证QAMS的可行性及其准确性。结果 22批荔枝多酚提取物特征指纹图谱标定了19个共有峰,指认了其中9个共有峰,即4个已知主成分6号峰(原花青素B2)、8号峰(表儿茶素)、9号峰(PC-C)、15号峰(原花青素A2),3个A型原花青素三聚体(12、16、17号峰),1个A型原花青素二聚体(19号峰)和1个B型原花青素二聚体(14号峰);22批荔枝多酚提取物相似度大于0.9,其中4个主成分的QAMS计算值与外标法实测值间无显著差异(P>0.25)。结论指纹图谱与QAMS结合的质控模式准确可行,可为全面合理评价荔枝多酚提取物的质量提供参考。
Objective To evaluate the polyphenol extracted from Litchi chinensis and quantify the content of four kinds of polyphenol therein, the combination of fingerprint and quantitative analysis of multi-components by single marker（QAMS） was used. MethodsA total of 22 batches of extract from Litchi chinensis were assayed by RP-UPLC to establish a common mode of fingerprints. For achieving QAMS, a method was developed by selecting epicatechin as internal reference and the relative correction factor of the three components, procyanidin A2, procyanidin B2, and epicatechin-（4β→8,2β→O→7）-epicatechin-（4β→8）-epicatechin（PC-C）, to determine their contents. The feasibility and accuracy of QAMS were evaluated by comparing the contents of four polyphenols determined with two different methods, QAMS and external standard method. Results Nineteen common peaks were identified in the characteristic fingerprint, nine components, including the known principal components, procyanidine B2（peak 6）, epicatechin（peak 8）, PC-C（peak 9）, procyanidine A2（peak 15）, three trimers of procyanidine type A（peaks 12, 16, and 17）, a dimer of procyanidine type A（peak 19） and a dimer of procyanidine type B（peak 14）, were verified in 22 batches of Litchi chinensis extract. Good similarities with correlation coefficients higher than 0.9 were found in 22 batches fingerprints. There was no significant difference between calculated value and detected value of the four ingredients in 22 batches, by QAMS and external standard method. Conclusion The results showed that the combined method of fingerprint and QAMS for quality control is accurate and feasible and provide reference method to evaluate the quality of extracts from Litchi chinensis.
出处
《中草药》
CAS
CSCD
北大核心
2017年第3期490-498,共9页
Chinese Traditional and Herbal Drugs
基金
广东省科技计划项目(2013B021800090)
