摘要
目的比较不同产地薏苡Coix lacrymajobi有效成分甘油三油酸酯和薏苡素量的差异。方法甘油三油酸酯的检测以Discovery C_(18)为色谱柱,乙腈-二氯甲烷(66∶34)为流动相,体积流量0.7 m L/min,柱温30℃,蒸发光散射检测器,漂移管温度45℃,氮气体积流量1.5 L/min;薏苡素检测采用HPLC法,以Agilent ZORBAX SB-C_(18)为色谱柱,乙腈-水(25∶75)为流动相,体积流量1 m L/min,柱温25℃,检测波长232 nm。结果浙江泰顺薏苡仁中甘油三油酸酯量最高,达到1.04%,质量分数最低的是安徽亳州产薏苡仁,为0.53%;上海产薏苡中的薏苡素量最高,达到3.469 7 mg/g,但安徽产薏苡中的薏苡素质量分数最低,为0.619 3 mg/g。结论 8个不同产地的薏苡中甘油三油酸酯和薏苡素的量均存在较大差异,其中浙江、福建2地产薏苡甘油三油酸酯和薏苡素量相对较高,药材品质较佳。
Objective To compare the differences of contents of effective components triolein and coixol in Coix lacryma-jobi var. mayuen from different origins. Methods The Discover C18 column was used for the detection of triolein with mobile phase of acetonitrile-dichloromethanol（66：34）. The flow rate of mobile phase was 0.7 m L/min. The column temperature was controlled at 30 ℃. The ELSD was used. The tube temperature was 45 ℃, and the flow rate of nitrogen was 1.5 L/min. The Agilent Zorbax SB-C18 column was used for the detection of the coixol with the mobile phase of acetonitrile-water（25︰75）, a flow rate of 1 m L/min, a temperature of 25 and a wavelength of 232 nm. ℃ Results The triolein content of Zhejiang province was the highest, at 1.04%, and the lowest was in Hainan Province, which was 0.53%; The highest coixol content was in Shanghai, reaching 3.469 7 mg/g, but the lowest content was only 0.619 3 mg/g in Anhui province. Conclusion The contents of triolein and coixol in adlay from eight Provinces are significantly different, the contents of triolein and coixol in adlay from Zhejiang and Fujian Provinces were relatively high, and medicinal material quality is better.
出处
《中草药》
CAS
CSCD
北大核心
2017年第3期578-581,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81473301)
关键词
薏苡
甘油三油酸酯
薏苡素
有效成分
HPLC
康莱特
Coix lacrymajobi L.var.mayuen Stapf
triolein
coixol
effective components HPLC
KLT
