摘要
以海带为原料,采用酶法破壁技术和固相萃取技术,研究纤维素酶、果胶酶及其组成的复合酶对海带多酚溶出效果的影响,并确定最佳工艺条件。结果表明,选用果胶酶进行酶解破壁,最佳工艺条件为:加酶量20 000 U·kg^(-1),酶解p H值5.0,酶解温度60℃,酶解时间90 min,海带多酚提取率达1.68 g/kg;经固相萃取纯化后,海带多酚的高效液相色谱(high performance liquid chromatography,HPLC)信号值提高了9.24倍,杂质峰明显减少,实现了海带多酚的高效纯化。
Bio-enzymes and solid phase extraction were used for the extraction of polyphenols from kelp. The extraction efficiency for polyphenol extracts from Laminaria japonica by using cellulase, pectinase and their compounds were evaluated and then the optimal technological conditions were determined. The results showed that the extraction efficiency of polyphenols was 1.68 g,/kg after pectinase enzyme hydrolyzing the cell walls of Laminaria japonica ; and the optimum parameters of enzyme hydrolyzing were as follows ： enzyme hydrolyzing for 90 min, 2000 U · kg-1, pH 5.0, 60 ℃, the signal value of high performance liquid chromatography （HPLC） was improved to 9.24 times and the impurity peaks were significantly reduced after solid phase extraction being employed.
出处
《集美大学学报（自然科学版）》
CAS
2017年第1期21-28,共8页
Journal of Jimei University：Natural Science
基金
福建省自然科学基金资助项目(2015J01139)
福建省省属高校科研专项基金资助项目(JK2016021)
福建省中青年教师教育科研项目(JAT160249)
福建省高校自然基金重点项目(JZ160448)
关键词
海带
多酚
生物酶
固相萃取
Laminaria japonica
polyphenols
bio-enzymes
solid phase extraction
