摘要
目的:观察清毒伸筋汤对胶原诱导性关节炎大鼠血清肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)的影响,从分子水平探讨清毒伸筋汤治疗类风湿关节炎的作用机理。方法:60只大鼠随机选取10只为正常组,其余大鼠采用弗氏不完全佐剂和牛II型胶原蛋白诱导大鼠类风湿关节炎模型,模型制作成功后随机分为模型组,甲氨蝶呤组,清毒伸筋汤高剂量组、中剂量组、小剂量组。各治疗组灌服相应药物,正常组和模型组给予生理盐水。28 d后,各组小鼠主动脉采血,采用酶联免疫吸附法检测各组大鼠TNF-α、IL-1β水平。结果:清毒伸筋汤各剂量组均能降低大鼠血清TNF-α、IL-1β的水平,其中高剂量组、中剂量组与模型组比较,差异有统计学意义(P<0.05或P<0.01)。清毒伸筋汤中剂量与甲氨蝶呤组比较,差异无统计学意义(P>0.05),两者效果相当。结论:清毒伸筋汤能降低类风湿关节炎大鼠血清中TNF-α、IL-1β水平,从而抑制炎症反应,进一步阻止骨质破坏。
Objective ：To observe the effect of Qingdu Shenjin Decoction on serum tumor necrosis factor-α（TNF-α） and interleukin- 1β（IL-1β） of rats with collagen-induced Arthritis and to explore the mechanism of Qingdu Shenjin Decoction in treatment of Rheumatoid Arthritis in molecular level. Methods ： Sixty rats were randomly selected as normal group. Rat models of Rheumatoid Arthritis were induced by Freund incomplete adjuvant and bovine type 11 collagen. The model rats were randomly divided into model group, Methotrexate group, high-dose Qingdu Shenjin Decoction group, middle-dose Qingdu Shenjin Decoction group and low-dose Qingdu Shenjin Decoction group. The rats in each treatment group were fed with the corresponding drugs, while rats in normal group and model group took saline. 28 days later,blood was collected from aorta of each mouse and the levels of TNF-α and IL-1β was examined with enzyme-linked immune sorbent assay in the aorta of each group. Results：The levels of TNF-α and IL-1β in the serum of rats treated with Qingdu Shenjin Decoction were significantly lower than those of the model group （P 〈 0. 05 or P 〈0. 01 ）. There was no significant difference in levels of TNF-α and IL-1β between the middle-dose Qingdu Shenjin Decoction group and the methotrexate group （P 〉 0.05 ）. Conclusion ： Qingdu Shenjin Decoction can decrease the levels of TNF-α and IL-1β in the serum of rats with rheumatoid arthritis, so as to inhibit the inflammatory reaction and further prevent the bone destruction.
出处
《中医学报》
CAS
2017年第1期93-95,共3页
Acta Chinese Medicine
基金
广西科学研究与技术开发计划课题(桂科攻13349006)
