摘要
建立了基质固相分散萃取-毛细管电泳法检测米制品中丙烯酰胺的新方法。通过实验确定了最佳前处理条件:C18及弗罗里硅土混合作为萃取分散剂(质量比1∶4),样品与分散剂的质量比为5∶9,洗脱液为丙酮-二氯甲烷(体积比6∶4)的混合溶剂,洗脱剂体积为10 m L。在优化实验条件下,以20 mmol/L硼砂(p H 8.4)作缓冲溶液,在15 k V恒压下进行分离,该方法的线性范围为10~200μg/m L,相关系数(r)为0.999 8,丙烯酰胺的检出限为0.62μg/m L,样品中丙烯酰胺的回收率为85.7%~96.4%。
A new method coupling matrix solid phase dispersion（MSPD） with capillary electrophoresis was developed for the determination of acrylamide in rice product. The optimized conditions obtained in experiments were as follows： Florisil and C18 were used as a mixed dispersant with a mass ratio of 1 ∶ 4. The mass ratio of sample to dispersant was 5 ∶ 9. The mixture of 10 m L acetone-dichloromethane（6 ∶ 4） was used as elution solvent. Under the optimized experimental conditions,20 mmol/L borate solution（p H 8. 4） was used as buffer. The sample was separated at a constant voltage of 15 k V. The linear range of acrylamide was in the range of 10-200 μg/m L,with a correlation coefficient（r） of 0. 999 8. The limit of detection（LOD,S/N = 3） was 0. 62 μg/m L for acrylamide and the recoveries were in the range of 85. 7%-96. 4%.
出处
《分析测试学报》
CAS
CSCD
北大核心
2017年第2期280-283,共4页
Journal of Instrumental Analysis
基金
住建部科技计划项目(2013-K7-24)
关键词
丙烯酰胺
基质固相分散
毛细管电泳
米制品
acrylamide
matrix solid-phase dispersion（MSPD）
capillary electrophoresis
rice product
