摘要
试验旨在研究中药常山散的体外抑菌活性。分别采用试管二倍稀释法联合琼脂平板稀释法及营养琼脂稀释法对选用的12种致病菌进行抑菌试验,测定最低抑菌浓度(MIC),进行抗菌作用量效关系研究,并通过牛津杯法观察药物抑菌效果。试管二倍稀释法联合琼脂平板法结果表明,常山散对链球菌属和芽孢杆菌的抑菌效果较强,MIC在15.6~62.5mg/mL之间;对金黄色葡萄球菌和肠杆菌有较弱的抑菌作用,MIC在250~500mg/mL之间;对真菌的抑菌作用最弱,MIC值>500mg/mL,其抑菌强度大小依次为:链球菌属、芽孢杆菌、肠杆菌、真菌。营养琼脂稀释法结果表明,常山散对变形杆菌、白色念珠菌及黑曲霉具有一定的抑菌作用,MIC约为500mg/mL,而对其他链球菌属、肠杆菌属及芽孢杆菌属细菌的抑菌效果较弱,MIC值均>500mg/mL。牛津杯法抑菌活性研究结果显示,常山散药液(500 mg/mL)对12种菌均有一定的抑菌效果,但抑菌效果较弱,绝大多数抑菌环直径≤10mm。牛津杯周围可见明显的药物作用圈,作用圈内细菌数量较其他部位明显减少。综合以上试验结果,中药常山散对常见致病菌均具有一定抑菌效果,但由于药物本身的特性及有效组分含量较低,其抑菌作用效果较弱。
The purpose of this study was to indicate the antibacterial effect of traditional Chinese medicine Radix dichroa powder（RDP）in vitro.Minimal inhibitory concentration（MIC）of RDP for 12 pathogens were determined by two fold dilution method and agar dilution method,and the dose-effect relationship of antibiotic effect was studied,and the antimicrobial effect was also observed by Oxford-cup method.The results from two fold dilution method indicated that RDP had stronger inhibited effect to Streptococcus and Bacillus subtilis（MIC were from 15.6to 62.5mg/mL）than that of S.aureus（MIC was about 250 mg/mL）,Enterobacteria（MIC was about 500 mg/mL）and fungi（MIC500mg/mL）;The results of agar dilution method showed that RDP had some antibacte-rial activities on Proteusbacillus vulgaris,Candida albicans And aspergillus niger,MIC was about 500mg/mL,while it had weak antibacterial effect on other pathogens（MIC〉500mg/mL）.The results of Oxford-cup method showed that RDP（500mg/mL）had some degree antimicrobial effect on pathogens,and the bacteria-inhibiting ring diameter of RDP not exceeded 10 mm,while drug-action ring was observed obviously around the Oxford-cup,and the number of bacteria was significantly decreased within the ring.In conclusion,RDP had some degree antibacterial effect on common pathogens,but the effects were not strong because of charicteristics of RDP itself and lower effective content.
出处
《中国畜牧兽医》
CAS
北大核心
2017年第2期594-600,共7页
China Animal Husbandry & Veterinary Medicine
基金
中央级公益性科研院所专项资金项目(1610322011004)
横向委托项目(2015-007)
甘肃省农业生物技术研究与应用开发项目(GNSW-2013-29)
关键词
常山散
最低抑菌浓度
致病菌
抑菌试验
Radix dichroa powder（RDP）
minimal inhibitory concentration（MIC）
pathogen
bacteriostatic test
