摘要
从福建省某猪场采集的发病仔猪大脑和内脏病料中分离到一株病毒。病毒接种PK细胞的结果显示,PK细胞出现典型的细胞病变;PCR反应扩增出特异性的片段,与经典毒株闽A株一致;免疫荧光试验中出现绿色荧光;病毒接种家兔后引起典型的伪狂犬病症状并死亡。上述结果充分证实,本研究分离的毒株为伪狂犬病病毒,随之将其命名为伪狂犬病病毒FJNP株。本研究结果丰富了福建省伪狂犬病流行病学资料,为开发猪伪狂犬病病毒新型疫苗提供了依据。
A new virus strain was isolated from nosogenic piglets＇ brain and visceral disease material on a pig farm in Fujian.Experiments of inoculating the virus strain into PK cells,a polymerase chain reaction（PCR）,an immunofluorescence assay,and inoculating the strain into rabbits were carried out.In result,PK cells showed typical cytopathic,and a specific fragment was amplified from the strain,which was consistent with the classical virus strain A in Fujian.Green fluorescence appeared in the immunofluorescence assay,and the inoculated rabbits were died with typical pseudorabies clinical symptoms.The result confirmed fully that the virus strain was a new pseudorabies virus,then named the pseudorabies virus FJNP strain,which enriched the pseudorabies virus database of Fujian of China and provided the basis for development of new vaccines against pseudorabies virus.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2017年第2期207-211,共5页
Chinese Veterinary Science
基金
福建省自然科学基金项目(2014J01107)
福建省公益类项目(2015R1023-10)
福建省农科院牧医所基金项目(MYQJ2014(S)-2)
关键词
猪
伪狂犬病病毒
毒株
分离
鉴定
porcine
pseudorabies virus
strain
isolation
identification
