摘要
目的:对比观察钛基材料表面经等离子喷涂技术改性的钽、钛涂层对其生物特性的影响。方法:厚度为1mm的钛板,经喷砂、等离子钽喷涂和等离子钛喷涂表面改性,制备成直径1.5cm样品。分为钽涂层试件、钛涂层试件和喷砂钛试件共三组,样品无菌处理后,分别接种分离纯化的人牙周膜干细胞培养3天,扫描电镜观察各组材料表面细胞粘附和生长情况并拍片,采用Image J图形软件分析材料表面细胞被覆面积占比,评价材料的生物相容性。结果:与无细胞空白对照组比较,钽涂层、钛涂层及喷砂钛各组试件表面均有细胞粘附生长,但粘附细胞形态、数量、增殖及细胞状态有所不同。钽涂层试件组材料表面细胞在低倍镜下观察呈片状生长,突起链接形成雪花样,平均密度高于喷砂钛和钛涂层试件组,高倍镜见细胞在材料表面伸展充分,分泌基质样颗粒,并伸出伪足进入材料微孔内,Image J图像分析细胞面积平均百分比为33.05±1.48,显著高于喷砂钛试件组;钛涂层试件组材料表面也有细胞粘附生长,细胞形态与钽涂层试件组无明显差别,但细胞面积平均百分比为21.92±1.39,虽不及钽涂层试件组但明显多于喷砂钛试件组14.86±1.13;方差齐性检验显示钛涂层试件组与另两组试件的组间有差异(P<0.05),喷砂钛与钽涂层试件组间具有显著性差异(P<0.01)。三者占比面积结果显示:钽涂层试件组>钛涂层试件组>喷砂钛试件组。结论:等离子喷涂表面改性促进了钛基材料表面生物活性;等离子钽喷涂表面改性较钛涂层及钛喷砂处理具有更好的生物相容性。
Objective： To compare the effects of tantalum and titanium coating on the biological properties of titanium based materials. Methods： The titanium plates, 1 mm thickness, have been modified by sandblasting, plasma spraying on the surface, and preparation the specimen into 1.5 cm diameter disc.It is divided into three parts： tantalum coating specimen, titanium coated specimen and sandblasting titanium sample,. Moreover, we select 7 samples from each sets and aseptic technique, then respectively inoculated the human Periodontal ligament stem cells for 3 days. Scanning electron microscope display the adhering and growing conditions of the cells on all those materials, and analysis the material area covered by cells（ by SPSS22.0 Image J）, then evaluate the biocompatibility of the material. Results： Comparing with the cell-free controlling groups, the experimental group s all own the growth and adhesion of cells, but their morphology, quantity and velocity of proliferation of cells are different. At the low magnification, the cells on tantalum coating surface protuberant link snow pattern formation, the number is significantly higher than sandblasting titanium group. At the high magnification, the cells on tantalum coating surface stretch in the material surface, and secrete matrix sample particles, moreover, they extended pseudopodia into the microporous material. The mean values about the area percentage of cells is 33.05±1.48, significantly higher than that of sand blasting titanium group; Titanium material surface coating group also has the growth of the cells, and the cells morphology show no significant difference with tantalum coating group, but the area percentage of cells is 21.92±1.39, relatively less than tantalum coating group, but more than sandblasting titanium group. F test show that titanium coating group and the other 2 groups are different（P〈0.05）, sandblasting titanium and tantalum coating has a significant difference between groups（P〈0.01）. according to the results： tantalum coating grouptitanium coating group 〉sandblasting titanium group. Conclusion： Plasma spraying and surface modification could promote the biocompatibility of titanium base material. Plasma spraying tantalum coating surface modification has better biocompatibility than titanium coating and sandblasting processing.
出处
《中华老年口腔医学杂志》
2017年第1期27-32,共6页
Chinese Journal of Geriatric Dentistry
基金
国家高技术研究发展计划(863计划)(项目编号:2015AA033502)
全军后勤科研计划重大项目(项目编号:AWS14C007)
关键词
钽涂层
钛涂层
喷砂钛
等离子喷涂
人牙周膜干细胞
tantalum coating
titanium coating
sandblasting titanium
plasma-spraying technique
human periodontal ligament stem cells
