摘要
以野生型坛紫菜自由丝状体为培养材料,研究不同光质(绿光510~550 nm、蓝光455~475 nm、红光580~630 nm、白光400~760 nm)的发光二极管(LED)对无性增殖过程中自由丝状体生长及生理特性的影响。结果显示,蓝光能提高自由丝状体的生长速率,蓝光下藻体增重分别是白光、绿光、红光下藻体增重的1.10倍、1.82倍和2.17倍。蓝光处理有助于叶绿素a和类胡萝卜素的积累,二者含量分别较白光、绿光和红光处理提高13.77%、47.69%、63.42%和8.87%、87.07%、97.73%。蓝光和白光均有利于藻红蛋白合成,而绿光和红光降低藻红蛋白合成,但4种光质的LED光源对藻蓝蛋白合成的影响无显著性差异。蓝光能显著提高碳酸酐酶(CA)活性,较白光下CA活性增加11.36%。蓝光和绿光显著提高1,5-二磷酸核酮糖羧化酶/加氧酶(Rubis CO)活性,分别较白光下Rubis CO活性增加28.17%和61.21%。红光和绿光下藻体在培养后期的色泽暗淡,星状色素体少,部分藻体细胞内容物溢出,呈中空状态;而蓝光和白光下藻体健康正常,色泽鲜红。研究表明,在今后的坛紫菜自由丝状体无性扩繁过程中可以适当增加LED光源的蓝光组分,减少红光和绿光组分。
The influences of different light qualities（green 510-550 nm, blue 455-475 nm, red 580-630 nm, white400-760 nm） from light-emiting diode（LED） illuminations on the growth and physiological characteristics of wild free-living conchocelis of Pyropia haitanensis were studied. Results showed that blue light（BL） significantly promoted the growth rate of conchocelis in the cultures. The weight increments of conchocelis under BL were1.10, 1.82, 2.17 times higher than those of other conchocelis cultured under white light（WL）, green light（GL） and red light（RL）, respectively. BL stimulated the synthesis of chlorophyll a and carotenoids. Compared to WL, GL and RL, the contents of chlorophyll a in BL were increased by 13.77%, 47.69%, 63.42%, respectively, and the contents of carotenoids in BL were increased by 8.87%, 87.07%, 97.73%, respectively. BL and WL were conducive to synthesize phycoerythrin rather than GL and RL. However, there were no significant differences of phycocyanin contents between the conchocelis in the cultures under different LED light qualities. Moreover, BL resulted in the highest activity of carbonic anhydrase, which enhanced by 11.36% than those of WL. Compared to WL, both BL and GL significantly stimulated the activity of ribulose bisphosphate carboxylase, which enhanced28.17% and 61.21%, respectively. In the late growth stage, the conchocelis cultured under RL and GL were discolored and short of satellite chromatophore, even parts of them overflowed their cell inclusions and were hollowed. Whereas, conchocelis under BL and WL were normal, healthy and bright red. Therefore, we could add suitable component of blue-light, and decrease red-light and green-light in the asexual propagation of free-living conchocelis in P. haitanensis.
出处
《水产学报》
CAS
CSCD
北大核心
2017年第2期230-239,共10页
Journal of Fisheries of China
基金
福建省种业创新与产业化工程项目(2014S1477-10)
福建省自然科学基金(2014J01095)
福建省海洋高新产业发展专项(闽海洋高新2014-19)
福建省海洋与渔业结构调整专项
福建省海洋经济创新发展区域示范项目(2014FJPT01)
厦门南方海洋研究中心项目(14PZY017NF17
15GZY021NF04)~~
关键词
坛紫菜
自由丝状体
光质
生长
生理特性
Pyropia haitanensis
free-living conchocelis
light quality
growth
physiological characteristics
