摘要
以三倍体率、卵裂率、D幼率、生产成本等为指标,比较了CB、6-DMAP两种化学试剂诱导香港牡蛎三倍体的效果,研究了试剂浓度、诱导时机、诱导持续时间及受精卵密度等4种因素对香港牡蛎三倍体的诱导效应。结果显示,在温度28~30°C、盐度15~25,受精卵密度为2.0×10~8个/L条件下,采用0.5 mg/L的CB在受精后15~18 min处理,诱导持续时间为20 min,可产生100%三倍体;合子的卵裂率为53.16%~63.00%,D形幼虫孵化率为47.32%~53.09%,诱导效率指数为0.47~0.53,生产成本为260元/L。相同条件下,采用浓度为75~100 mg/L的6-DMAP处理,诱导持续时间为20~25 min,受精卵处理密度4.5×10~7个/L,可产生62.52%~72.36%的三倍体;合子的卵裂率为60.00%~66.25%,D形幼虫孵化率为74.43%~90.00%,诱导效率指数为0.47~0.65,生产成本为139~185元/L。综合比较两种方法,6-DMAP诱导方法更加适合用于大规模的香港牡蛎三倍体苗种生产。本研究为香港牡蛎多倍体育种提供了研究数据与实践经验。
Triploid of Crassostrea hongkongensis was induced by blocking the second polar body release by cytochalasin B（CB） and 6-dimethylaminopurine（6-DMAP）, and we compared the two methods in the aspects of triploid rate, cleavage rate, velar larvae gain, cost and so on. The effects of the concentration of CB or 6-DMAP,induced occasion, induced duration and zygote density on inducing triploid C. hongkongensis were also discussed.The results showed that： when oyster zygotes were fertilized at 28–30 °C and salinity at 15–25, and dealt with 0.50mg/L CB began at 15–18 min, post fertilization lasted 20 min, and incubated density of zygote when treatment was about 2.0×108 ind/L. These were optimum conditions under which triploid of C. hongkongensis was induced by CB. In this case, triploid yield was 100%, cleavage rate was 53.16%–63.00%, velar larvae gain was47.32%–53.09%, the efficiency of triploid induction was 0.47–0.53, and the cost was 260 RMB/L. The surrounding when fertilization, and induced occasion was the same as those in CB, but oyster zygotes were dealt with 75–100 mg/L 6-DMAP last 20–25 min, and incubated density of zygote when treatment was about 4.5×107ind/L. These were optimum conditions of triploid of C. hongkongensis was induced by 6-DMAP. In this case,triploid yield was 62.52%–72.36%, cleavage rate was 60.00%–66.25%, velar larvae gain was 74.43%–90.00%, the efficiency of triploid induction was 0.47–0.65, and the cost was 139–185 RMB/L. Through comprehensive comparison between CB and 6-DMAP, we could draw the conclusion that 6-DMAP was more applicable in triploid production of C. hongkongensis than CB. This study provided the basic theory and practical experience for polyploidy breeding in oyster.
出处
《水产学报》
CAS
CSCD
北大核心
2017年第2期250-257,共8页
Journal of Fisheries of China
基金
广东省科技厅项目(2013B020201002,2014B020202011)
国家贝类产业体系建设专项(CARS-48)~~
