摘要
目的：观察白鲜皮水提物对小鼠迟发型变态反应的影响。方法取体重为18～22 g的昆明小鼠60只，雌雄各半，按体重随机分6组，即空白对照组、模型对照组、白鲜皮水提物高（11．0 g／kg）、中（55．g／kg）、低（2．8 g／kg）剂量组和阳性对照（地塞米松片2．22 mg／kg）组，每组10只，各给药组分别给予相应浓度的相应药物，空白与模型对照组给予0．015 ml／g 80％吐温20-生理盐水溶液，每日1次，连续给药3 d后，除空白对照外，每只鼠腹部用5％DNCB溶液均匀涂抹进行致敏，第4 d强化一次，于给药后第9 d，将5％DNCB溶液均匀涂抹于小鼠右耳进行攻击，空白对照组同样涂耳但未致敏，攻击后24 h，测定耳肿胀度和小鼠的脾脏指数和胸腺指数。结果以耳肿胀度为指标，与模型组相比较，白鲜皮高剂量组、阳性对照组具有极显著性差异（P＜0．01），白鲜皮中剂量组具有显著差异（P＜0．05），白鲜皮低剂量组则无显著性差异；以胸腺指数、脾脏指数为指标，与模型组相比较，白鲜皮高剂量组、白鲜皮中剂量组、阳性对照组具有极显著性差异（ P＜0．01），白鲜皮低剂量组胸腺指数较模型组具有显著性差异（ P＜0．05）。结论白鲜皮水提物可抑制DNCB诱导的小鼠迟发型变态反应（ DTH）及抑制小鼠的特异性细胞免疫功能的作用。
Objective To observe the effect of the cortex dictamni on the delayed type hypersensitivity in mice.Methods 60 Kunming mice weighing 18~22g, half female and half male , were randomly and averagely divided into 6 groups according to weight：blank control group, model control group, the cortex dictamni（11.0g /kg）, in （5.5g /kg） and low （2.8g /kg） dose group and positive control group （dexamethasone tablets 2.22mg/kg） , 10 rats in each group , The corresponding concentrations of each drug group were given respectively , the blank and the model control group were given 0.015ml/g 2%Twain 80%saline solution, 1 times a day, after 3 days of continuous administration , in addition to the blank control , each mouse abdomen with 5%DNCB solution was evenly coated for sensitized , fourth days to strengthen once , in ninth days , the 5%DNCB solution evenly attack on mouse ear , the blank control group also painted ear but not sensitized , after the attack of 24h, determination of ear swelling degree and the index thymus and spleen .Results In ear swelling as an indicator , comparing cortex dictamni , high dose group and model group , positive control group with a very significant difference （P〈0.01）, cortex dictamni dose group were significantly different （P〈0.05）, cortex dictamni leather low-dose group had no significant difference; thymus and spleen indexes as an indicator , as compared with the model group cortex dictamni leather high-dose group , cortex dictamni dose group , positive control group with a very significant difference （P〈0.01）, cortex dictamni on the low-dose group compared with the model group , the thymus index had significant difference （ P〈0.05） .Conclusions The cortex dictamni can inhibit DNCB induced murine delayed hypersensitivity （ DTH） and has the effect of inhibiting the specific cellular immune function of mice.
出处
《齐齐哈尔医学院学报》
2016年第30期3741-3742,共2页
Journal of Qiqihar Medical University
基金
黑龙江省自然科学基金(D201207)
关键词
白鲜皮
迟发型变态反应
Cortex Dictamni
Delayed type hypersensitivity
